Re: [ccp4bb] Spots not getting indexed properly in protein-DNA complex

2013-08-01 Thread Vandna Kukshal
Hi , Seems to me that crystal packing is not good thats why mosaic diffraction, this might be due to DNA so you can increase diffraction quality by using 1 or 2 nucleotide sticky end DNA for crystallization which can help in crystal packing . Vandna Kukshal Postdoctral Research Associate De

Re: [ccp4bb] Spots not getting indexed properly in protein-DNA complex

2013-08-01 Thread Bosch, Juergen
Hi Appu, nothing really to worry about if you process your data with XDS or d*trek using 3D profile fitting. You still should be able to get something useful out of this data. If you look at 90.png (I'm attaching a zoomed area of your image), you see your crystal is either split or you had two c

Re: [ccp4bb] Spots not getting indexed properly in protein-DNA complex

2013-08-01 Thread Nicolas Foos
Dear Appu, one direction of your cristal has very anistropic diffraction. I think your cristal has grown like multi-layer. The problem is probably due to the DNA. If the different "layer" of your cristal are not very well aligned, you have this type of problem. In my opinion, you can try to

Re: [ccp4bb] Spots not getting indexed properly in protein-DNA complex

2013-08-01 Thread Toth, Eric
Where Discovery Transforms Medicine From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Appu kumar Sent: Thursday, August 01, 2013 9:45 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] Spots not getting indexed properly in protein-DNA complex Dear all ccp4 user, We have col