[ccp4bb] TEV protease alternatives and Proteases which leave little on new C-term

2014-04-28 Thread Keller, Jacob
Dear Crystallographers (this may be off-topic, depending on whom you ask...) A: Can anyone recommend new improved alternatives to the usual suspects for proteases (TEV, thrombin, enterokinase, etc.)? I've seen some literature about SUMO- and NEDD8-dependent enzymes, but those apparently require

Re: [ccp4bb] TEV protease alternatives and Proteases which leave little on new C-term

2014-04-28 Thread David Blum
Dear Jacob, We have been using factor XA for several years for tag removal of the proteins we express in the facility. Factor XA does not leave an overhang, cutting after an IEGR sequence. It can be quite expensive though so we are looking into methods to purify it from bovine plasma. David