Dear Crystallographers (this may be off-topic, depending on whom you ask...)
A: Can anyone recommend new improved alternatives to the usual suspects for
proteases (TEV, thrombin, enterokinase, etc.)? I've seen some literature about
SUMO- and NEDD8-dependent enzymes, but those apparently require
Dear Jacob,
We have been using factor XA for several years for tag removal of the
proteins we express in the facility. Factor XA does not leave an overhang,
cutting after an IEGR sequence. It can be quite expensive though so we are
looking into methods to purify it from bovine plasma.
David
