Subject: Re: [ccp4bb] Unexplained density near cobalt
To: CCP4BB@JISCMAIL.AC.UK
Date: Thursday, July 7, 2011, 9:39 PM
Could be a hexacoordinated cobalt with a water molecule (or a hydroxyl ion)
depending on the chemical environment... Artem
On Thu, Jul 7, 2011 at 10:07 AM, Machius, Mischa
Hi MM,
Co in its +2 oxidation state typically forms a tetrahedrally coordinated
species in high pH environment; and pentagonally coordinated species with five
ligands at low pH. A Co ion in its +3 oxidation state forms a octahedrally
coordinated species with six ligands. Co +3 is unusual in
hey Mischa
I would guess that is a split cobalt/metal site occupancy 0.1 and 0.9 or
something like that.
If you calculate an anomalous difference map you may be able to confirm/reject
that suggestion, depending on the strength of the anomalous signal.
cheers
Preben
On 07.07.2011, at
We have a manganese binding protein that binds two Mn ions in a binuclear
complex. It turns out that one of the metal ions can move about 2.0 Å
depending on crystallization data collection conditions (check out PDB files
1ON1 and 2F5D for the alternate conformations). In some instances we
Could be a hexacoordinated cobalt with a water molecule (or a hydroxyl ion)
depending on the chemical environment...
Artem
On Thu, Jul 7, 2011 at 10:07 AM, Machius, Mischa Christian
mach...@med.unc.edu wrote:
Y'all,
I was wondering if anyone had any thoughts about a feature we observe with
