hari jayaram schrieb:
...
I was wondering if anyone could comment on combining datasets from
multiple P1 crystals to increase the redundancy even further for such
heavy atom ( SAS / SAD ) or MAD experiments.
Hari,
well, my comment would be that it should be possible in principle from
what
Increasing redundancy only helps if all data draw from the same
distribution so you get a more accurate estimate of the mean of the
distribution. When dealing with different crystals, crystal-to-crystal
variation is likely larger than the anomalous signal you are looking for
and I'm therefore
]
***
- Original Message -
From: Bart Hazes [EMAIL PROTECTED]
To: CCP4BB@JISCMAIL.AC.UK
Sent: Wednesday, July 23, 2008 10:05 AM
Subject: Re: [ccp4bb] Using multiple crystals for structure solution in P1
using MAD/SAS/SAD
Increasing redundancy only helps if all data
: 773.608.9185
email: [EMAIL PROTECTED]
***
- Original Message - From: Bart Hazes [EMAIL PROTECTED]
To: CCP4BB@JISCMAIL.AC.UK
Sent: Wednesday, July 23, 2008 10:05 AM
Subject: Re: [ccp4bb] Using multiple crystals for structure
solution in P1 using MAD
]
***
- Original Message - From: Bart Hazes [EMAIL PROTECTED]
To: CCP4BB@JISCMAIL.AC.UK
Sent: Wednesday, July 23, 2008 10:05 AM
Subject: Re: [ccp4bb] Using multiple crystals for structure solution
in P1 using MAD/SAS/SAD
Increasing redundancy only helps if all data draw from the same
distribution
]
***
- Original Message - From: Bart Hazes [EMAIL PROTECTED]
To: CCP4BB@JISCMAIL.AC.UK
Sent: Wednesday, July 23, 2008 10:05 AM
Subject: Re: [ccp4bb] Using multiple crystals for structure solution in P1
using MAD/SAS/SAD
Increasing redundancy only
We are faced with phasing a structure for a protein that refuses to
crystallize in any spacegroup but P1.
To add to the fun , the resolution for most selenomethionine and heavy atom
soak datasets ranges from 3.8 to 4.2 A .
In order to increase the redundancy we have been taking many inverse
