: 02 February 2010 22:34
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Vapor diffusion calculator
Dear Crystallographers,
Is anybody aware of a calculator for vapor diffusion experiments to
plot
concentrations of various solvent components as a function of time?
For
a
simple
I would like to add that contrary to popular belief, MPD would have to be
treated as a volatile in the example you give below. It vaporizes, although
very slowly, as can be proven by leaving a 1uL drop of it in open air for a
couple of days.
Cheers,
Jose Antonio Cuesta Seijo.
Jacob Keller
tricks will
have to be applied though.
Cheers,
Boaz
- Original Message -
From: Jacob Keller j-kell...@md.northwestern.edu
Date: Wednesday, February 3, 2010 0:34
Subject: [ccp4bb] Vapor diffusion calculator
To: CCP4BB@JISCMAIL.AC.UK
Dear Crystallographers,
Is anybody
regardless of what such a (kinetic) calculator will calculate at the end -
we will have to feed it with data of the type vapour pressure vs.
concentration and temperature of substance X. Even without a whole
simulation tool for the kinetics of a vapour diffusion experiment this
will already
Hi,
this might be relevant here:
http://scripts.iucr.org/cgi-bin/paper?S0907444995000436
Imre
Clemens Grimm wrote:
regardless of what such a (kinetic) calculator will calculate at the
end -
we will have to feed it with data of the type vapour pressure vs.
concentration and temperature of
Zitat von Imre Toeroe t...@embl.de:
Hi,
this might be relevant here:
http://scripts.iucr.org/cgi-bin/paper?S0907444995000436
Yes! thanks for the link.
Imre
Clemens Grimm wrote:
regardless of what such a (kinetic) calculator will calculate at the end -
we will have to feed it with data
: 773.608.9185
email: j-kell...@northwestern.edu
***
- Original Message -
From: Imre Toeroe t...@embl.de
To: CCP4BB@JISCMAIL.AC.UK
Sent: Wednesday, February 03, 2010 4:25 AM
Subject: Re: [ccp4bb] Vapor diffusion calculator
Hi,
this might be relevant here
Dear Crystallographers,
Is anybody aware of a calculator for vapor diffusion experiments to plot
concentrations of various solvent components as a function of time? For a
simple example, what happens when I mix a protein solution containing 50mM
NaCl 1:1 with a reservoir containing 50% MPD
Good Idea!
You would have to specify volume and surface area for the two aqueous
phases, and maybe volume of the vapor phase. To keep it simple
assume diffusion within each phase is fast compared to transfer
between, so you don't develop gradients within the liquid phase and
unstirred layer
