[ccp4bb] advances in multisubunit membrane protein

2017-02-03 Thread Fulvio Saccoccia, Sapienza
Dear ccp4ers, I was wondering about most recent advances in production and crystallization of complexes of multisubunit membrane proteins, for instance succinate dehydrogenase, pyruvate dehydrogenase complex or even photosystem complex. As far as I know, many large complexes of membrane pr

Re: [ccp4bb] advances in multisubunit membrane protein

2017-02-03 Thread Edward A. Berry
Speaking specifically for succinate dehydrogenase, there are a number of assembly factors required for insertion of flavin, iron-sulfur clusters (SDHAF1,2,3 . . .). Since E. coli or Pischia make their own SDH, there is a possibility the endogenous assembly factors and co-factors would work with t