Hi Alex,
Such gels were used to separate in the past 70S ribosome, plus/minus one
protein - so separation is excellent. Cannot recall the references but
papers published in the 70s' I believe.
The agaose is used to provide "strength" to low percentage acrylamide, say
3% acrylamide/0.5% agarose. J
:[EMAIL PROTECTED] On Behalf Of Dima
Klenchin
Sent: Sunday, August 24, 2008 11:34 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] agarose-acrylamide composite gels
>Sorry for the off-topic question. I am trying to separate by SDS-PAGE
>really big proteins (>500 kDa), and lower percen
Sorry for the off-topic question. I am trying to separate by SDS-PAGE
really big proteins (>500 kDa), and lower percentage (3-8%) acrylamide
gels do not do the trick. Based on literature searches, acrylamide-agarose
composite gels seem the way to go. Is anyone willing to share a protocol?
I can
Hello everyone:
Sorry for the off-topic question. I am trying to separate by SDS-PAGE really
big proteins (>500 kDa), and lower percentage (3-8%) acrylamide gels do not do
the trick. Based on literature searches, acrylamide-agarose composite gels seem
the way to go. Is anyone willing to share a
