Re: [ccp4bb] agarose-acrylamide composite gels

2008-08-25 Thread Daniel Pomeranz Krummel
Hi Alex, Such gels were used to separate in the past 70S ribosome, plus/minus one protein - so separation is excellent. Cannot recall the references but papers published in the 70s' I believe. The agaose is used to provide "strength" to low percentage acrylamide, say 3% acrylamide/0.5% agarose. J

Re: [ccp4bb] agarose-acrylamide composite gels

2008-08-24 Thread Artem Evdokimov
:[EMAIL PROTECTED] On Behalf Of Dima Klenchin Sent: Sunday, August 24, 2008 11:34 AM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] agarose-acrylamide composite gels >Sorry for the off-topic question. I am trying to separate by SDS-PAGE >really big proteins (>500 kDa), and lower percen

Re: [ccp4bb] agarose-acrylamide composite gels

2008-08-24 Thread Dima Klenchin
Sorry for the off-topic question. I am trying to separate by SDS-PAGE really big proteins (>500 kDa), and lower percentage (3-8%) acrylamide gels do not do the trick. Based on literature searches, acrylamide-agarose composite gels seem the way to go. Is anyone willing to share a protocol? I can

[ccp4bb] agarose-acrylamide composite gels

2008-08-24 Thread Alexandra Deaconescu
Hello everyone: Sorry for the off-topic question. I am trying to separate by SDS-PAGE really big proteins (>500 kDa), and lower percentage (3-8%) acrylamide gels do not do the trick. Based on literature searches, acrylamide-agarose composite gels seem the way to go. Is anyone willing to share a