Re: [ccp4bb] and another MR problem

2010-08-23 Thread Teresa De la Mora
Dear all The P21 unit cell is a=86.499 b=38.554 c=94.027 al=90.0 be=97.92 ga=90.0 The C2 unit cell is a=103.932 b=62.253 c=96.305 al=90.0 be=111.934 ga=90.0 Phaser and the other programs do give me solutions but the LLG in Phaser is about -1000's and the highest TFZ score I get is 4.9, and

Re: [ccp4bb] and another MR problem

2010-08-23 Thread Roger Rowlett
When doing MR, I usually try Phaser and EPMR. Phaser rarely fails for a high-homology MR search, but can have difficulty fitting 3 or more protein units in the ASU. EPMR is a little better, in my experience, in ferreting out a solution for 3 or more protein units

[ccp4bb] and another MR problem

2010-08-23 Thread Teresa De la Mora
Dear all I have a molecular replacement problem and I really need your help with this one. My protein crystallized in C2 instead of P21 like it did before. Now when I tried to do MR to place it in C2 it doesn't work, I get no solution. This protein has been crystallized in P212121, P31, P