Re: [ccp4bb] b-factors from refmac tls refinement vs cns mlf refinement

2007-06-13 Thread Eleanor Dodson
These are B factors RELATIVE to your TLS parameters. You need to run tlsanl to get back to something comparable to the individual B factors.. Eleanor CRAIG MCELROY wrote: Hi all, I'm working on the structure of a heterodimer phased by molecular replacement using data to 2.9 angstroms. After

[ccp4bb] b-factors from refmac tls refinement vs cns mlf refinement

2007-06-13 Thread CRAIG MCELROY
Hi all, I'm working on the structure of a heterodimer phased by molecular replacement using data to 2.9 angstroms. After MR I performed a rigid body refinement in CNS, if I then refine using mlf in CNS I get B-factors ranging from ~37-119 and r= 0.26 free_r= 0.35 whereas if I perform a TLS rest