A small comment: crystals that do not diffract well at R.T. can still
diffract well when frozen. There are several reasons for this, including:
* poor stability of crystal once the drop is open to the air
* crystal has low tolerance for handling (capillary mount can be
challenging and cause mor
them
habituated to the cryo.
Shane Atwell
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On
Behalf Of Jenny
Sent: Wednesday, April 04, 2007 5:50 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] bigger size - > better diffraction?
Hi, All,
I got a crystal that diffracts
habituated to the cryo.
Shane Atwell
-Original Message-
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On
Behalf Of Jenny
Sent: Wednesday, April 04, 2007 5:50 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] bigger size - > better diffraction?
Hi, All,
I got a crystal that diffra
in getting them
habituated to the cryo.
Shane Atwell
> -Original Message-
> From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On
> Behalf Of Jenny
> Sent: Wednesday, April 04, 2007 5:50 AM
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [ccp4bb] bigger size - > better diff
Hello Jenny,
0.2 Rmerge may be an indication of incorrectly assigned SG or perhaps a
misindexing by one, etc. Check what happens if you reprocess in P4.
For a 10kDa protein, your unit cell is kind of large - depending of course
on the symmetry, the higher obviusly the better. I would bet that you
oh, One thing forgot to mention is that I actually collected data in
house ( about 70 frames ), the completness is high ( 98%) but the
Rmerge is high ( 0.2 ), what does this suggest?
On 4/4/07, Jenny <[EMAIL PROTECTED]> wrote:
Hi, All,
I got a crystal that diffracts at 3.3A in house.The crystal
Hi, All,
I got a crystal that diffracts at 3.3A in house.The crystal size is
about 0.2mm* 0.1mm * 0.2mm. At first I thought the size is fine,but it
turns out the smaller ones diffract worse.I guess the reason is that
the cell unit is really big (126.292 126.292 134.904 p4212,
pretty big for
