: [ccp4bb] co-express two proteins in E.coli
I have been using the Duet system from Novagen (or whatever it is called
these days), specifically the pETDuet-1 and pRSFDuet-1. Co-expression of
my proteins did not work in either vector. Either, one protein expressed
or the other. I played around
Dear ALL,
We are planning to co-express two proteins in E.coli.
Could anyone suggest a good dual set plasmid or a proper insertion
sequence between two genes, including the Shine-Dalgarno sequence?
Thank you very much and have a nice summer.
Jerry McCully
I've had success with pet-Duet.
http://ecoliwiki.net/colipedia/index.php/pETDuet-1
Jason.
--
Jason Busby
PhD Student
Laboratory of Structural Biology
School of Biological Sciences
University of Auckland
Thomas Building 110
3a Symonds St
Private Bag 92019
Auckland 1142
New Zealand
ph: +64 9
I have been using the Duet system from Novagen (or whatever it is called these
days), specifically the pETDuet-1 and pRSFDuet-1. Co-expression of my proteins
did not work in either vector. Either, one protein expressed or the other. I
played around with the promotors (they are both T7) by
I have been using the Duet system from Novagen (or whatever it is called
these days), specifically the pETDuet-1 and pRSFDuet-1. Co-expression of
my proteins did not work in either vector. Either, one protein expressed
or the other. I played around with the promotors (they are both T7) by
-
From: Dima Klenchin klenc...@facstaff.wisc.edu
To: CCP4BB@JISCMAIL.AC.UK
Sent: Tuesday, July 17, 2012 5:16:34 AM
Subject: Re: [ccp4bb] co-express two proteins in E.coli
I have been using the Duet system from Novagen (or whatever it is called
these days), specifically the pETDuet-1 and pRSFDuet-1
