Re: [ccp4bb] cryocrystals

2013-05-22 Thread James Holton
Theoretically? Millions to billions of years. Practically? They will last until the first time you forget to fill the dewar. Or until the Sun explodes and in the rush to leave Earth your descendants forget to pack it. Whichever comes first. Seriously, as long as the crystals have actual

Re: [ccp4bb] cryocrystals

2013-05-22 Thread Mark J van Raaij
in theory I would say decades/eons. in practice it probably depends on how much water/ice your liquid nitrogen contains, multiple refillings of the liquid nitrogen might slowly deposit tiny ice crystals on the crystals and slowly make diffraction worse... Mark J van Raaij Lab 20B Dpto de Estruc

Re: [ccp4bb] cryocrystals

2013-05-22 Thread Enrico Stura
careina Cryocrystals will last several months and more. Make sure that your LN2 is dry. On Wed, 22 May 2013 14:38:51 +0200, Careina Edgooms wrote: Hi Does anybody know how long one could store a crystal in liquid nitrogen for before it will no longer diffract well? I'm talking in the or

Re: [ccp4bb] cryocrystals

2013-05-22 Thread Bosch, Juergen
I dare say indefinitely. Your crystal life expectancy drops rapidly though with somebody not remembering to fill up the dewar. Transferring crystals to pucks etc. is another problem. I had some crystals which I could not remember what they were (label broke off the cane) and we simply reshoot th

[ccp4bb] cryocrystals

2013-05-22 Thread Careina Edgooms
Hi Does anybody know how long one could store a crystal in liquid nitrogen for before it will no longer diffract well? I'm talking in the order of weeks to months... careina