Dear all,
The question is regarding the cryoprotectant.We have crystallized a protein
in 20% PEG 500 with 10% isopropanol.What should be the cryo we should try
for data collection.Also,what percentage of PEG 500 is sufficient enough as
a cryo for data collection.
We are also trying to crystallize
Not that all proteins are created alike, but we had a protein
crystallize in a similar situation (PEG/20%IPA). I don't remember the
exact details, but we did have good luck using just glycerol as a
cryoprotectant. That is, I made up the well buffer and added to that
well buffer 20-25%
(lab)
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
shivesh kumar
Sent: Wednesday, March 19, 2008 3:34 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] cryoprotectant for crystals in isopropanol
Dear all,
The question is regarding
