I believe octanol (I don't know where the -ol has to be) has been used the
same manner - i.e. saturate the octanol with ligand and overlay the drop.
This was reported at the ccp4 study weekend on protein complexes (possibly
by Annie Hassell at GSK???)- so more info can probably be found in Acta D
I've just remembered another approach that I've heard suggested,
although I don't know anyone who has tried it.
Dissolve plenty of the small molecule in paraffin or silicone oil, and
use this mixture to cover the drop. This can be used with regular vapor
diffusion or (probably better) micro
Hi,
I am used to have the same problem with hydrophobic ligands, in special
due to the rupture of crystals solely by the solvent even in tiny amount. So, I
decided to mix a suspension of the ligand with the crystallization drop (once
assured I can distinguish the protein from ligand). In
There's also NMP (N-methyl-2-pyrrolidone).
-- Ian
> -Original Message-
> From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On
> Behalf Of Green, Todd
> Sent: 22 January 2007 20:40
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [CCP4BB] crystal friendly solvents
You can try DMI (1,3-dimethyl-2-imidazolidinone) as an alternative to
DMSO. Also, sometimes you get lucky, and the small molecule is more
soluble when high concentrations of glycerol are present, so you can
do the soak in a cryo buffer...
On Jan 22, 2007, at 3:40 PM, Green, Todd wrote:
H
