Re: [ccp4bb] getting weak diffracting crystals

2008-12-05 Thread Debajyoti Dutta
uL of >the well solution to see their effect. > >good luck, >Mathews > > > > > From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Debajyoti > Dutta >Sent: Thursday, December 04, 2008 9:04 PM >To: CCP4BB@JISCMAIL.AC.UK

Re: [ccp4bb] getting weak diffracting crystals

2008-12-04 Thread Debajyoti Dutta
  Hi again, Thank you for your reply. I know that the loop regions are notorious for crystallization however in this case it is yielding the crystals but with lower quality. Does it not due to the quick crystallization, the lattice has no time to form up. That’s why it is happening. Can I n

Re: [ccp4bb] getting weak diffracting crystals

2008-12-04 Thread artem
Dear Deb, We've seen the detrimental effects of local disorder time and tme again. So - yes it is very likely that the putative disordered loops detrimentally affect the quality of your crystals. You can try to engineer your protein to be better - it usually takes a number of internally engineere

[ccp4bb] getting weak diffracting crystals

2008-12-03 Thread Debajyoti Dutta
  Dear Members, I am getting crystals of my protein. The secondary structure prediction implies that it has N-terminal with high degree of loop regions. I also get some mountable crystals yielding weak diffraction pattern(10 A). The quality of the crystals can also be assumed from its texture