Re: [ccp4bb] help with the structures

2011-11-22 Thread Rajesh kumar
Subject: Re: [ccp4bb] help with the structures I think you are proving yet again that refinement at 3.3A is not easy. Indeed there are probably multiple conformations for parts of the structure and that may well be why your data is at low resolution and anisotropic. Maybe this is the best you

Re: [ccp4bb] help with the structures

2011-11-22 Thread Eleanor Dodson
...@hotmail.com CC: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] help with the structures I think you are proving yet again that refinement at 3.3A is not easy. Indeed there are probably multiple conformations for parts of the structure and that may well be why your data is at low resolution and anisotropic

Re: [ccp4bb] help with the structures

2011-11-21 Thread Eleanor Dodson
I think you are proving yet again that refinement at 3.3A is not easy. Indeed there are probably multiple conformations for parts of the structure and that may well be why your data is at low resolution and anisotropic. Maybe this is the best you can do.. I think I would make sure the apo

[ccp4bb] help with the structures

2011-11-19 Thread Rajesh kumar
Dear All, We have an anisotropic dataset of 3.3 A and it was solved (not by me) with P6522 with R/freeR 29.1/37.3. I got the corrected mtz file by plugging in the .HKL (P6122) file to anisotropy diffraction server at 2.04 A. I reindexed this p6122 to p6522 and extended the resolution