t, BR
>
>
>
> *From:* CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] *On Behalf Of *
> Mattiroli,Francesca
> *Sent:* Tuesday, February 17, 2015 5:23 AM
> *To:* CCP4BB@JISCMAIL.AC.UK
> *Subject:* [ccp4bb] how to reduce protein solubility
>
>
>
> Hi all,
>
>
[est...@cea.fr]
Sent: 20 February 2015 14:36
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] how to reduce protein solubility
Ursula,
Most compounds used for cryosolutions glycerol, ethylene glycol, propane
diol increase protein solubility.
A warning, these compounds are also hygroscopic, you nee
Ursula,
Most compounds used for cryosolutions glycerol, ethylene glycol, propane
diol increase protein solubility.
A warning, these compounds are also hygroscopic, you need to change your
vapour diffusion methodology.
Vera L., Czarny B., Georgiadis D., Dive V., Stura E.A. (2011) Practical
U
To: CCP4BB@JISCMAIL.AC.UK
Cc:
Subject: [ccp4bb] how to reduce protein solubility
Hi all,
I am struggling with a protein complex that is too soluble. I have reached
about 20 mg/ml but I still observe very little precipitation (clear drops in
90-95% of the tested conditions). The proteins are expressed in in
.
Alun
Sent from my Samsung Galaxy smartphone.
Original message
From: "Mattiroli,Francesca"
Date:17/02/2015 04:34 (GMT+00:00)
To: CCP4BB@JISCMAIL.AC.UK
Cc:
Subject: [ccp4bb] how to reduce protein solubility
Hi all,
I am struggling with a protein complex that is too
Thank you, everyone!
Francesca
From: Enrico Stura [est...@cea.fr]
Sent: Tuesday, February 17, 2015 3:00 AM
To: CCP4BB@jiscmail.ac.uk; Mattiroli,Francesca
Subject: Re: [ccp4bb] how to reduce protein solubility
Francesca,
The most common failure is to have
Francesca,
The most common failure is to have an excessive amount of salt (salting
in/ salting out), glycerol or other solubilizing
ingredient in your protein solution. I would suggest that you change the
pH and reduce the salt in your protein solution,
by microdialysis if you do not have mu
Mattiroli,Francesca
Sent: Tuesday, February 17, 2015 5:23 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] how to reduce protein solubility
Hi all,
I am struggling with a protein complex that is too soluble. I have reached
about 20 mg/ml but I still observe very little precipitation (clear drops in
On Behalf Of David
Briggs
Sent: 17 February 2015 08:12
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] how to reduce protein solubility
Hi Francesca,
Try some zinc (1mM + ) in your protein buffer? Zinc tends to make a lot of
things less soluble in my hands.
Dave
On Tue, 17 Feb 2015 04:34 Mattiro
Hi Francesca,
Try some zinc (1mM + ) in your protein buffer? Zinc tends to make a lot of
things less soluble in my hands.
Dave
On Tue, 17 Feb 2015 04:34 Mattiroli,Francesca <
francesca.mattir...@colostate.edu> wrote:
> Hi all,
>
> I am struggling with a protein complex that is too soluble. I h
ruary 16, 2015 11:23 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] how to reduce protein solubility
Hi all,
I am struggling with a protein complex that is too soluble. I have reached
about 20 mg/ml but I still observe very little precipitation (clear drops in
90-95% of the tested conditio
Bringing the pH closer to the measured pI would definitely help. The pI can
be measured on an IEF gel. Glycosylation, if applicable, could dramatically
increase solubility.
Roger Rowlett
On Feb 16, 2015 11:33 PM, "Mattiroli,Francesca" <
francesca.mattir...@colostate.edu> wrote:
> Hi all,
>
> I a
Hi all,
I am struggling with a protein complex that is too soluble. I have reached
about 20 mg/ml but I still observe very little precipitation (clear drops in
90-95% of the tested conditions). The proteins are expressed in insect cells
and going to higher concentration is not easily achievable
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