Hi Anita,
Try to lower the ph of binding buffer if your protein allows also you may
lower the concentration of salt like nacl if adding into the buffer also
try to reduce the imidazole concentration of your eluted fraction gradually
by performing serial dialysis before SEC. Alternatively, you can
Hello Crystallographers,
I am trying to express and purify a soluble domain of a membrane protein
for crystallization. The amino acid content is as below
Ala (A) 12 13.8% Arg (R) 10 11.5% Asn (N) 2 2.3% Asp (D) 8 9.2% Cys (C) 1
1.1% Gln (Q) 1 1.1% Glu (E) 4 4.6% Gly (G) 16 18.4% His (H) 3 3.4%
