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From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
shivesh kumar
Sent: 05 March 2008 07:23
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] isopropanol as a precipitant
Dear all
Sorry for non-CCP4 query.
I have
Hi, if you look at papers on crystallization of the E.coli protein ROP,
you might find interesting info, in particular e.g.:
Papanikolau Y, Kotsifaki D, Fadouloglou VE, Gazi AD, Glykos NM, Cesareni G,
Kokkinidis M.
Ionic strength reducers: an efficient approach to protein purification and
Dear all
Sorry for non-CCP4 query.
I have crystallized a 7kDa protein in 50-60% of isopropanol,pH
4.0-4.6.Theinteresting thing is that the xtal appears within 5 hrs at
16 degree.
The protein conc is 5mg/ml and drop size is 3+1 with mother liquor 300 micro
lt.Numerous xtals appears and but small is
