Good (?) afternoon,
I can't resist quoting the facetious answer, once given to me in a similar
situation by the dear departed Felix Frolow: '*proteins are bastards*'.
Well to be exact he used a much less socially acceptable term, and he said
it in Russian - but the point is that sometimes (most of
Thank you all for responses. I have added my reply below.
On 26 October 2016 at 17:02, Eleanor Dodson wrote:
> Are your 3 crystals isomorphous, and do they all refine well?
Yes, all C2221 crystal datasets refine to ~ 19/23 %
> I guess you struck lucky with DS3 - as suggested it could be a differ
Fiorentino
Sent: 26 October 2016 13:32
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] ligand binding and crystal form
Hello all,
I just solved a NCS-tetrameric (biological assembly is just a dimer) crystal
structures with ligand soak (same plate - same conditions). No density for
ligand is observed in
What is the height of non-origin Patterson function peak for your data sets?
C-centered cells:
216.5 345.8 145.290.090.090.0
and
147.0 354.3 217.490.090.090.0
are very different; however, they have common subgroup F222 with similar
unit cell parameters. In F
Are your 3 crystals isomorphous, and do they all refine well?
I guess you struck lucky with DS3 - as suggested it could be a different
soaking, different crystal size, etc, etc..
If isomorphous I would compare all 3 structures in COOT and look for subtle
differences..
Your C222 and C2221 cells a
Are these three crystals in order of harvesting (with different soaking times)?
How big is your ligand. How accessible is the binding pocket (and is there a
clear difference in accessibility between chains)?
T
Tristan Croll
Research Fellow
Cambridge Institute for Medical Research
University
Hi Valentina,
> I just solved a NCS-tetrameric (biological assembly is just a dimer) crystal
> structures with ligand soak (same plate - same conditions). No density for
> ligand is observed in the first map. In the 2nd, I have 1 ligand bound. In
> the 3rd, I have 2 ligands bound. Is there any
Dear Veronica,
with 1st, 2nd, 3rd map you mean the density for the same dataset after
three consecutive cycles of building-refining or three different maps from
three different crystals?
If it's the first case, it could be fine, it may mean that at each cycle
you improve the map so you see signal
Hello all,
I just solved a NCS-tetrameric (biological assembly is just a dimer)
crystal structures with ligand soak (same plate - same conditions). No
density for ligand is observed in the first map. In the 2nd, I have 1
ligand bound. In the 3rd, I have 2 ligands bound. Is there any reason for
this
