As always, Dale hits the nail on the head.
Your R factor is a function of your scaling algorithm as well as your
model, and as you point out the apparent R factor increases when you
include the low resolution data. This doesnt mean your model is worse -
it is still the same model!
All
Hi all,
during refinement of our (membrane protein) structures, basically in all cases
the R/Rfree values depend a lot on the low resolution cutoff. Putting the
cutoff at lower res (20-50 A) results in substantially higher R/Rfree values
(sometimes few percent). For this reason we mostly
Hi Bert,
try phenix.refine: it uses very efficient and robust bulk solvent
correction and anisotropic scaling protocol (Acta Cryst. (2005). D61,
850-855) as well as it automatically detects and removes
reflections-outliers before the refinement starts. I think the
combination of these things
Are you using CNS 1.2? This version has a robust bulk solvent model and
anisotropic correction
that is much improved compared to CNS 1.1. It is similarly robust as
that of Phenix (although
different in detail).
Axel Brunger
Van Den Berg, Bert wrote:
Hi all,
during refinement of our
: [ccp4bb] low-res cutoff in refinement
Are you using CNS 1.2? This version has a robust bulk solvent model and
anisotropic correction
that is much improved compared to CNS 1.1. It is similarly robust as
that of Phenix (although
different in detail).
Axel Brunger
Van Den Berg, Bert wrote
Subject: Re: [ccp4bb] low-res cutoff in refinement
Are you using CNS 1.2? This version has a robust bulk solvent model and
anisotropic correction
that is much improved compared to CNS 1.1. It is similarly robust as
that of Phenix (although
different in detail).
Axel Brunger
Van Den Berg, Bert wrote
