Dear Frank,
It is difficult to say something without the diffraction images but probably
they are detergent ( or lipid) crystals. These crystals are in general soft
and don't diffract well.
My suggestion is to know well what your crystals really are before any
optimization.
Best,
Isabel
: Wednesday, October 23, 2013 5:22 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] membrane protein optimization
Hi CCP4BB Forks,
In recently I got a membrane protein crystal in the quite normal
membrane protein crystallization conditions as other persons reported,
like PEG400 16-19%, pH 6.0-7.5, with 50
Hi Frank,
unfortunately it is very common with membrane protein crystals to get stuck
with diffraction quality around 20A.
From what you describe you could consider the following:
a) revise the detergent you solubilize your MP with (e.g. use OG instead of
DDM), and consider to change to
Hi Frank,
Do not forget that membrane protein crystals are often fragile and
difficult to manipulate.
Finding good cryo condition can be difficult and small temperature
variation can destroy crystals
within minutes, this makes room temperature diffraction tests not always
obvious. The time
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of crystalboy
[yyb...@gmail.com]
Sent: Wednesday, October 23, 2013 11:22 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] membrane protein optimization
Hi CCP4BB Forks,
In recently I got a membrane protein crystal in the quite
] membrane protein optimization
Hi CCP4BB Forks,
In recently I got a membrane protein crystal in the quite normal
membrane protein crystallization conditions as other persons reported,
like PEG400 16-19%, pH 6.0-7.5, with 50 mM MgCl2 (in my case) by using
sitting drop method. These crystals
