I also recommend you to check Dan's link on the paper for stabilising
protein solubility.
I have similar experience. I got high yield, soluble protein but
aggregates once I have purified concentrated protein.
Before venturing to the detergent area, I would suggest using other
additives
Going in a different direction with my reply here.
Is your pI close to your current buffer ? Move at least one unit away from the
theoretical pI.
Do you have a friend with a real time pcr machine ? Then get some sypro orange
and check out the thermal stability of your protein under various
Hi,
Sorry for off-topic question.
Does anyone have experience of the stabilisation of water-soluble proteins
by detergents? Protein I'm working with is definitely water-soluble and has
high yield, but, unfortunately, not very stable. Especially during
concentration. So, we thought that adding
: [ccp4bb] off-topic: detergents for the stabilisation of water-soluble
proteins
Hi,
Sorry for off-topic question.
Does anyone have experience of the stabilisation of water-soluble proteins by
detergents? Protein I'm working with is definitely water-soluble and has high
yield, but, unfortunately
The following paper (which can be found at
www.wolfson.huji.ac.il/purification/PDF/Literature/Bondos2003.pdf
Detection and prevention of protein aggregation before, during, and after
purification. Sarah E. Bondos and Alicia Bicknell (2003) Analytical
Biochemistry, 316, 223-231
contains a
Vitali,
Echoing what Dan said, I am not sure why you have chosen detergents first, as
there are many other agents which stabilize proteins. Is the instability due
to hydrophobic surfaces (e.g., made worse at higher salt) or not. Some
non-detergent suggestions are:
1) diols like MPD (see
