If you have a DLS you can try playing with different [NaCl] or pH and see if
you can push the equilibrium in one direction.
Jürgen
On Apr 6, 2012, at 11:41 AM, 杨贝 wrote:
Dear All,
I have a 100KD protein which elutes as a mixture of 90% monomer and 10% dimer
on HPLC, if I pool the monomer frac
Dear All,
I have a 100KD protein which elutes as a mixture of 90% monomer and 10%
dimer on HPLC, if I pool the monomer fractions and reload them onto the
column, the elution profile is still 90% monomer plus 10% dimer, and the
same is true if I do so to the dimer peak. Collectively, there seems
