Re: [ccp4bb] oof topic: pH effect on substrate analog

2012-10-31 Thread Ed Pozharski
On Tue, 2012-10-30 at 16:12 +, Peter Hsu wrote: I'm wondering, since I lack activity at this pH point, would it lead to no binding of a substrate analog? Not necessarily. You should check pH dependence of the Km - it might be that lower activity is primarily due to reduction in kcat.

[ccp4bb] oof topic: pH effect on substrate analog

2012-10-30 Thread Peter Hsu
Hi all, I'm working on a protein that I recently got crystals of. My functional studies show that the protein has optimal activity at lower pHs, while losing 90% activity at about pH8. I've been trying to soak/cocrystallize a substrate analog (small molecule) into my crystals (grown at ~pH8)

Re: [ccp4bb] oof topic: pH effect on substrate analog

2012-10-30 Thread Herman . Schreuder
, October 30, 2012 5:13 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] oof topic: pH effect on substrate analog Hi all, I'm working on a protein that I recently got crystals of. My functional studies show that the protein has optimal activity at lower pHs, while losing 90% activity at about pH8

Re: [ccp4bb] oof topic: pH effect on substrate analog

2012-10-30 Thread Chittaranjan Das
...@u.washington.edu To: CCP4BB@JISCMAIL.AC.UK Sent: Tuesday, October 30, 2012 12:12:58 PM Subject: [ccp4bb] oof topic: pH effect on substrate analog Hi all, I'm working on a protein that I recently got crystals of. My functional studies show that the protein has optimal activity at lower pHs, while

Re: [ccp4bb] oof topic: pH effect on substrate analog

2012-10-30 Thread Roger Rowlett
To: CCP4BB@JISCMAIL.AC.UK Sent: Tuesday, October 30, 2012 12:12:58 PM Subject: [ccp4bb] oof topic: pH effect on substrate analog Hi all, I'm working on a protein that I recently got crystals of. My functional studies show that the protein has optimal activity at lower pHs, while losing 90% activity