You may want to check out the Studier paper on autoinduction from 2005. It has
various bits of advice about SeMet labelling, like getting the amino acid
mixture right and vitamin/metal stocks. I follow this and do fine with BL21 DE3
cells.
Jon
On Thu, Jul 03, 2014 at 10:00 PM, Maher Alayyoubi
Hi Everyone,
Can someone please help me figure out what's wrong with my SelMet
incorporation protocol. My protein of interest is 500 amino acids with 20
of these being Methionines. However mass spec data show that only two of
these 20 Methionine sites are partially labelled with SelMet. The follow
