Dear ccp4bb members,
Sorry for asking a non-crystallographic question where google search cannot
provide any valuable answer.
pppGpp was synthesized by relQ enzyme using ATP and GTP, the product was
purified by anion exchange chromatography. The problem is that the
concentration of
desiccator with P2O5 in the freezer should do the trick...
Artem
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On Thu, Jun 11, 2015 at 11:12 PM, luzuok luzuo...@126.com wrote:
Dear ccp4bb members,
Sorry for asking a non-crystallographic question where google search
cannot provide any valuable
Can you elute from AE with NH4HCO3? It's volatile, so you could just evaporate
it off afterwards.
JPK
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of luzuok
Sent: Friday, June 12, 2015 7:13 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] ppGpp purification and concentrate