[ccp4bb] ppGpp purification and concentrate

2015-06-11 Thread luzuok
Dear ccp4bb members, Sorry for asking a non-crystallographic question where google search cannot provide any valuable answer. pppGpp was synthesized by relQ enzyme using ATP and GTP, the product was purified by anion exchange chromatography. The problem is that the concentration of

Re: [ccp4bb] ppGpp purification and concentrate

2015-06-11 Thread Artem Evdokimov
desiccator with P2O5 in the freezer should do the trick... Artem - Cosmic Cats approve of this message On Thu, Jun 11, 2015 at 11:12 PM, luzuok luzuo...@126.com wrote: Dear ccp4bb members, Sorry for asking a non-crystallographic question where google search cannot provide any valuable

Re: [ccp4bb] ppGpp purification and concentrate

2015-06-11 Thread Keller, Jacob
Can you elute from AE with NH4HCO3? It's volatile, so you could just evaporate it off afterwards. JPK From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of luzuok Sent: Friday, June 12, 2015 7:13 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] ppGpp purification and concentrate