Hello all
I am working on small protein-protein complex of 10 kDa each component.Among
these complex one of the partner known to be highly
flexible but in complex they are suppose to be well structured and from a
tight complex . I am trying to crystallise this complex. When i add the
protein in
Hi Peter,
You first want to reduce the protein concentration to avoid rapid
precipitation upon adding crystallization solutions. Try concentrations
of 5 - 10 mg/mL instead of 15 mg/mL. Next, consider changing the NaCl
concentration. Salt can act as a co-precipitant, and lowering its
concentrati
Given that you are able to achieve a protein concentration of 15 mg per ml
in Tris-HCl, pH 8.0, 100mM NaCl and 1% Glycerol suggests that you have no
drastic solubility problem.
Unless you have a chromatophore as an intrinsic co-factor of one of your
component proteins, the yellow slime may be due
