to a very good SAXS review article.
>
> Cheers,
> Angela
>
>
>
>
> Li Sheng
> Sent by: CCP4 bulletin board
> 04/23/2009 06:19 AM
> Please respond to
> Li Sheng
>
>
> To
> CCP4BB@JISCMAIL.AC.UK
> cc
>
> Subject
> [ccp4bb] protein
Since the SAXS signal comes from the size of your scattering
particles, make sure you don't have large non-protein particles in
your sample (dust or micelles, for example). The SIBYLS web site has
additional warnings about including detergents in your sample.
ho
UC Berkeley
To
CCP4BB@JISCMAIL.AC.UK
cc
Subject
[ccp4bb] protein purity in SAXS
Dear all,
Is there anyone with experience in small angle x-ray scattering? Could
anyone please tell me the requirements for protein sample purity and
concentration?
Thanx in advance.
Sincerely,
Li
3, 2009 1:19 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] protein purity in SAXS
Dear all,
Is there anyone with experience in small angle x-ray scattering? Could
anyone please tell me the requirements for protein sample purity and
concentration?
Thanx in advance.
Sinc
Dear Li,
the samples should be devoid of aggregates, at least for the low
concentration samples. Some aggregation can be tolerated at the higher
concentrations. You should in principle measure samples with different
concentrations, such as 1, 2, 5,10 mg/mL.
The high concentration measurements w
ent: Thursday, April 23, 2009 1:19 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] protein purity in SAXS
Dear all,
Is there anyone with experience in small angle x-ray scattering? Could
anyone please tell me the requirements for protein sample purity and
concentration?
Thanx in advance.
Sincer
Dear all,
Is there anyone with experience in small angle x-ray scattering? Could
anyone please tell me the requirements for protein sample purity and
concentration?
Thanx in advance.
Sincerely,
Li
__
Email:s...@sibs.ac.cn
Institute of Biochemistry and Ce
