Dear all, I wonder if there is anyone who ever tried composite omit map with low resolution data.
Is there any resolution limit for the composite omit map? or any criteria could help deciding the practicability, like quality of data, map or resolution? I have ~3.7A resolution data and MR solution (~40% Rfree), obtained from the two partial structures, reported before with 2.2A and 1.8A respectively. After extensive iterative refinement the Rfree is close to ~30% and each subunits shows noticeable conformational difference within complex. I am considering the composite omit map to test whether this difference comes is real or comes from model bias during refinement. Do you think it's a reasonable idea with this resolution? If not, any suggestion for this purpose? Thanks in advance for sharing your valuable time. Kyuwon Baek, Ph.D
