[ccp4bb] question on merging multiple data

2012-07-16 Thread Fengyun Ni
Hi all, I have to merge several datasets from different crystals because the crystals suffer from severe radiation damage. I read some previous posts and follow the protocol as described, 1) individually process the data; 2) scaleit to compare the Rfactors between different datasets; 3)

Re: [ccp4bb] question on merging multiple data

2012-07-16 Thread Eleanor Dodson
All this is best done from the GUI - pointless will sort out batch numbers, check indexing etc.. But you still have to identify any rogue batches, and decide on when to jettison the data st.. The Sigma level is related to the number of observations of each reflection, so this will decrease as

Re: [ccp4bb] question on merging multiple data

2012-07-16 Thread Fengyun Ni
Thank you, Eleanor! I'll try pointless. In my case, the Sigma increase as i merge all the five data while I/sigma decrease, which is opposite to what you describe. Is this abnormal? Fengyun Quoting Eleanor Dodson eleanor.dod...@york.ac.uk: All this is best done from the GUI - pointless