One or more ingredients in the original hit (which you use to suspend the
seed crystals in) can indeed be essential, and may be all you need to get
nice crystals. In D'Arcy's original paper you will see that he needed Ca2+
to get nice crystals of one of his proteins. The same is true in
Dear Mahesh,
cross-seeding into other conditions often works but still the chances
are much lower than seeding the same form.
Best wishes,
Matthias
(Please desist from sending so large attachments to half the planet!)
-
Dr. Matthias Zebisch
Division
Mahesh, this is a very interesting and slightly controversial question.
One approach is to mix together all of the crystals that you have in the
initial screen. The idea at the beginning of the project is to get as many
diverse hits as possible - you can worry about crystal size, space group
and
Thanks you for the suggestions, Patrick and Matthias. I was actually
wondering if any of the components from the seeding solution actually were
important but your explanations sound more logical.
I apologize for the large attachment. I did not realize that it was so big.
Have a nice weekend !
