Hi Swati,
I am very far from expert, but if you have anisotropic diffraction have
you thought of bringing in Staraniso - public server at
https://staraniso.globalphasing.org? That should help keeping
reflections with higher I/sigma and rejecting those with mostly noise.
Yours,
Rasmus
On 23/
Thanks for the suggestions.. actually I have been trying for quite some
already and this has lots of solvent channels ..hexagonal crystal with p62
group..not coming to a better resolution with completeness but am trying to
grow better and also trying to work out the data I already have
On Sun, 25
If the I/sigI and CC1/2 that you quote are for the outer resolution shell
of your data, you can use data to a higher resolution limit. It may still
be tricky, and rebuilding will be worse; as Eleanor says you'll probably be
better off growing a better crystal.
Good luck!
--
Kevin Jude, PhD (he/hi
Finding a solution will be very difficult then.
Eleanor
On Fri, 23 Apr 2021 at 21:11, Swati Gupta wrote:
> My data is moderate anisotropic also with a high Wilson b factor greater
> than 200
>
> On Sat, 24 Apr, 2021, 01:19 Eleanor Dodson,
> wrote:
>
>> If you were lucky the new crystal might ha
My data is moderate anisotropic also with a high Wilson b factor greater
than 200
On Sat, 24 Apr, 2021, 01:19 Eleanor Dodson,
wrote:
> If you were lucky the new crystal might have the same cell and
> spacegroup as your model, but otherwiseThis is a case for molecular
> replacement.?
>
> My cours
If you were lucky the new crystal might have the same cell and
spacegroup as your model, but otherwiseThis is a case for molecular
replacement.?
My course of action using CCP4I2
Process data carefully and look for any warnings.
Align your new sequence with the model using clustalw
Edit the model
Dear all,
how do I proceed with solving a protein structure with 3.9
A resolution and an i/sig i of 1.3 and cchalf of 0.8. The homologous
protein has 48% identity to be used as template. Kindly help with what
programs to run.
Thanks
Swati
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