We probably need more detail to help you.
Have you looked carefully at the data processing? Is the Rmerge or Rpim
reasonable for all batches? Is there any suggestion of twinning? Does the
wilson plot look linear? (These hexagonal SGs can be twinned)
How many copies of your molecule do you expect
Dear Deepak,
I guess that the spacegroup in the MTZ file that you use for refinement is
wrong.
I think you should carefully check the output of PHASER, and in particular the
PDB file that it wrote. The correct spacegroup is given there (and it may also
be P4122 or P4222 or P4322, because like
Dear all,
Greetings from Munich. I hope everything is well with you. I am writing to
take input on a problem related to the structure solution of a
protein-ligand complex.
I have crystallized a protein (7.6kDa) with a ligand (5.2kDa). The crystal
diffracted to *2.71 Angstron*, and the data were p
