Are the crystals growing as a result of the skin, or coincidentally located
with the skin? For instance, if you are using the hanging drop method, and
the crystals are growing with the skin because of their density, you could
switch to sitting drop plates -- crystals grow on the bottom, skin stays
Hello Everyone,
Thank you for all the suggestions on how to manipulate skin on crystal
drops. Here is a summary of responses:
(i) Higher concentrations of reducing agents (ii) Layering crystal drop with
cryo-protectant (iii) Trypsinize the skin (iv) Grow crystals under oil (v)
Try 4C if crystals
