Hello, we want to use the thermofluor thermal shift assay to screen for buffers that our proteins are happy in.
Reading around the subject, I am a bit unclear as to what to be looking for with regard to a stabilizing affect. Initially, we thought, the higher the Tm, then the more stabilizing the buffer is, but reading further we have observed several examples where, the Tm is raised, but yet the Delta-Tm indicates a destabilizing affect. How can this be so? Any references regarding a good explanation of Delta-Tm would be great. thanking you Andy Dodds
