Dear Peter,
it's a common phenomenon to create protein concentration gradients inside a
protein concentrator. Simply take it all out of the concentrator, put it in
a separate tube, and mix thoroughly/vortex. The 'slime' may very well
redissolve and you'll a homogeneous distribution. What you des
day, July 02, 2009 10:53 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] very high concentration of protein
Hello all
I am working with a small protein-protein complex. This complex express
quite well . I purify in a buffer of pH=9.0 with 150mM NaCl and 1% of
glycerol and able to concentrate u
Hello all
I am working with a small protein-protein complex. This complex express
quite well . I purify in a buffer of pH=9.0 with 150mM NaCl and 1% of
glycerol and able to concentrate upto 20 mg per ml. I have a two clones of
this protein complex. One is N-terminal His tagged and another C-termin
