Hello,
I usually try out Hampton crystal screen I & II, and PEG/ion screens
on my proteins, sometimes varying protein concentrations and
incubation temperature. If no hints are obtained, I will go back to
test alternative constructs and purification protocols. With more and
more companies selling crystal screens, and the accumulation of
knowledge from structural genomics projects, I worry that my practice
might have been outdated. I wonder if people could share with me:
1) What is your favorite set of crystal screens that maximize the
chance of getting the crystals while minimize the sample usage, and
perhaps more importantly, when do you decide to stop for better
constructs?
2) Are there any other screens targeting nucleic acid and NA-protein
complexes other than the Natrix and the Nucleic Acid Mini Screen sold
by Hampton Research? Here I mean real different conditions, not just
a re-label.
I'd appreciate your thoughts.
Best
Ailong
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