Dear Patrick,
You along with others had made some suggestions last time. May be its a good
time to update.
With classical screening, I got a crystal like appearances/shower with HEPES
7.5 and LiSo4 1.5M. Trying to vary the pH of Hepes or using Tris and with
different conc of Lithium I could
mitegen loops might help, particularly micromesh...
JPK
On Fri, May 11, 2012 at 12:35 PM, Rajesh Kumar ccp4...@hotmail.com wrote:
Dear Patrick,
You along with others had made some suggestions last time. May be its a
good time to update.
With classical screening, I got a crystal like
It sounds as though microseeding worked very well, but the crystals are
still growing far too quickly.
Try diluting the protein and/or the reservoir solution to half the concs
you are using or lower.
To have enough protein you need larger drops, say 500 + 500 + 200 by hand
if you can't do it
