20 percent (v/v).
Steve
> 2,3-BD users,
>
> What sort of concentrations are you using?
>
> Dave
>
> --
> Delivered via an Android.
>
> On Mar 22, 2010 11:45 PM, "Steve Tuske" wrote:
>
> Hi Ulrike,
>
> 2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it
> mostly seems to de
I would not rely on recipes. When I pick a cryo protectant, I always prepare a
set of solutions and take an image from a large loop dipped into solution.
Then I pick the one with the least concentration that does not show ice rings
anymore.
The behaviour can change with a crystal in the loop, but
Hello Dave,
I have good experience with concentrations between 10% and 15%
2,3-butanediol. My typical starting concentration is 12.5%.
Good luck.
Eike
Am 23.03.10 08:27 schrieb "David Briggs" unter :
> 2,3-BD users,
>
> What sort of concentrations are you using?
>
> Dave
>
> --
> Delive
2,3-BD users,
What sort of concentrations are you using?
Dave
--
Delivered via an Android.
On Mar 22, 2010 11:45 PM, "Steve Tuske" wrote:
Hi Ulrike,
2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it
mostly seems to decrease mosaicity. I was able to switch from a
thr
Hi Ulrike,
2,3-butanediol has worked very nicely as a cryoprotectant. In my hands it
mostly seems to decrease mosaicity. I was able to switch from a
three-step soaking protocol with glycerol and glucose to a quick (30
second to one minute) soak with 2,3-BD.
It's a winner.
Steve
I
> Hello Ulr
Hello Ulrike,
in our lab 2,3-Butanediol has had a good reputation.
Tim
On Mon, Mar 22, 2010 at 01:32:33PM +, Ulrike Demmer wrote:
> Hi everybody,
>
> has anyone experience with Butandiol and its different isoforms as
> cryoprotectant ?
>
> Thanks,
>
> Ulrike
--
--
Tim Gruene
Institut