Dear Eleanor,
sorry for the late reply. I found a similar case (id: 2OEF, Cys 92). I
mutated the Cys to Ser, but it did not change anything (xtal conditions and
quality remained the same). In solution the protein is a monomer.
However, adding 1 mM DTT resulted in a significant increase in crystal
Hello Eleanor,
We found some intermolecular vicinal disulfides recently that we think are
'real'. This class of proteins forms tetramers and in one version we find these
intermolecular disulfides across molecules. We did some tests and found that
oxidation or reduction has an effect on the
Does this count as an example?
grep SSBOND /a/pdb/pdb4e9m.ent
SSBOND 1 CYS A 39CYS B 39 1555 1555 2.05
SSBOND 2 CYS C 39CYS D 39 1555 1555 2.04
SSBOND 3 CYS E 39CYS F 39 1555 1555
Thank you for all this information, and especially Paul for having a good
search query - I had got nothing useful from any I tried at PDBe or RSCB.
No idea why it has been formed - the fold is identical to a high resolution
example (1.3A) where the disulphide links the N & C termini within a
I solved a structure years ago (1L31 and a couple related entries) which had
intermolecular oligomerizing disulfides in some of the crystals (reciprocal
C23-C27’ and C23’-C27). I thought at the time that they seemed too “deliberate”
to be just an artifact, but others thought that since the
