4, VAT Reg. GB 480 7371 36
--
*From:* CCP4 bulletin board [mailto:[EMAIL PROTECTED] *On Behalf Of *Green,
Todd
*Sent:* 22 January 2007 20:40
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* crystal friendly solvents that are useful for dissolving
hydrophobic small molecules?
Hello All,
I am trying to soak
94, VAT Reg. GB 480 7371 36
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of
Green, Todd
Sent: 22 January 2007 20:40
To: CCP4BB@JISCMAIL.AC.UK
Subject: crystal friendly solvents that are useful for dissolving
hydrophobic small molecules?
Hello All,
I am trying to soak
Hi,
I am used to have the same problem with hydrophobic ligands, in special
due to the rupture of crystals solely by the solvent even in tiny amount. So, I
decided to mix a suspension of the ligand with the crystallization drop (once
assured I can distinguish the protein from ligand). In
There's also NMP (N-methyl-2-pyrrolidone).
-- Ian
> -Original Message-
> From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On
> Behalf Of Green, Todd
> Sent: 22 January 2007 20:40
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: [CCP4BB] crystal friendly solvents
You can try DMI (1,3-dimethyl-2-imidazolidinone) as an alternative to
DMSO. Also, sometimes you get lucky, and the small molecule is more
soluble when high concentrations of glycerol are present, so you can
do the soak in a cryo buffer...
On Jan 22, 2007, at 3:40 PM, Green, Todd wrote:
H
Sorry Guys,
I did mean Dimethyl Formamide and not flouride. I apologize, I was thinking of
the ATP anologs thread as I was typing the response.
Sarathy
--
Notice: This e-mail message, together with any attachments, con
On Monday 22 January 2007 04:57 pm, Maneesh Yadav wrote:
> I've noticed that the iodobenzene does largely disappear overnight (in
> hanging drop), I don't know if this is because of evaporation or the
> iodobenzene just "falls" into the reservoir. Maybe stick to sitting drop.
Or it partitions thro
The method
below sounds very promising to me.
Mark
-Original Message-
From: [EMAIL PROTECTED]
To: CCP4BB@JISCMAIL.AC.UK
Sent: Mon, 22 Jan 2007 3:57 PM
Subject: Re: crystal friendly solvents that are useful for dissolving
hydrophobic small molecules?
So I've never actu
12:40 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: crystal friendly solvents that are useful for dissolving hydrophobic
small molecules?
Hello All,
I am trying to soak some crystals with a small molecule that is quite
hydrophobic. I am having trouble with solubilty of the small molecule. It will
diss
: Monday, January 22, 2007 3:40 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: crystal friendly solvents that are useful for dissolving
hydrophobic small molecules?
Hello All,
I am trying to soak some crystals with a small molecule that is quite
hydrophobic. I am having trouble with solubilty of the small
bject:* crystal friendly solvents that are useful for dissolving
hydrophobic small molecules?
Hello All,
I am trying to soak some crystals with a small molecule that is quite
hydrophobic. I am having trouble with solubilty of the small molecule.
It will dissolve up to about 1 mM in
Do you mean N,N-dimethylformamide, aka dimethylformamide, aka DMF, or do I
need a chemistry lesson?
On Monday 22 January 2007 02:49 pm, Parthasarathy, Gopalakrishnan wrote:
> Hi Todd,
> DMF (Dimethyl Fluoride) is a good alternative to DMSO.
>
> Sarathy
--
Craig A. Bingman, Ph.D.
Center for
useful for dissolving
hydrophobic small molecules?
Hello All,
I am trying to soak some crystals with a small molecule that is quite
hydrophobic. I am having trouble with solubilty of the small molecule.
It will dissolve up to about 1 mM in 100 % DMSO, but precipitates at
concentrations of less
Hello All,
I am trying to soak some crystals with a small molecule that is quite
hydrophobic. I am having trouble with solubilty of the small molecule. It will
dissolve up to about 1 mM in 100 % DMSO, but precipitates at concentrations of
less than 15 micromolar when the DMSO concentration is b
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