Re: [COOT] Refining Ramachandran outliers

2022-12-27 Thread Dr. Kevin M Jude
Hi Kate, if you're unable to get these outliers to fit, you might consider that there's a bigger problem like a register error, cis-peptide, incorrect backbone tracing, etc. If you have high resolution structures available for comparison if can be helpful. Best wishes Kevin Sent from my phone

[COOT] Refining Ramachandran outliers

2022-12-27 Thread Radford, Katelyn M. (Kate)
Hello! I'm working on refinement for a cryoEM structure at 3.4 Å. For this resolution, I'm aiming for 0 Ramachandran outliers, because I just don't have the density to defend their existence. Through iterative refinements in Phenix Real Space Refine and Coot, I've been able to get my Ramachandr

[COOT] 0.9.8.6 Carbohydrate linking issue still

2022-12-27 Thread Kelvin Lau
Dear Paul I looked back the forum and similar to Taka's issue in 0.9.8.4 I am also experience it with both Coot 0.9.8.6 EL (ccp4 build) and 0.9.8.5 (ccpEM) build. MacOS 12.6.1 It seems very similar to Taka's issue where if I add an NAG using the Carbohydrate module, I get my NAG, but no link.