Re: [Denovoassembler-users] Ray is crashing for no apparent reason

2013-12-06 Thread Sucheta Tripathy
Thanks Sebastian! I tried fixing that as well! Now both my libraries have good quality equal number of sequences: Here is the content of NumberOfSequences file: Files: 2 FileNumber: 0 FilePath: /scratch/sucheta/out1.fastq NumberOfSequences: 69393976 FirstSequence: 0

Re: [Denovoassembler-users] Ray on x86 seg fault: Address not mapped (Vertex::addRead)

2013-12-06 Thread MacManes, Matthew
Does this segfault happen right after section of optimal reads? Matt _ Matthew MacManes, Ph.D. University of New Hampshire I Assistant Professor Department of Molecular, Cellular, & Biomedical Sciences Durham, NH 03824 Phone: 603-862-4052 I Twitter: @Pero

Re: [Denovoassembler-users] Ion Torrent + Illumina Hybrid Assembly on Ray

2013-12-06 Thread Hornung, Bastian
I have only one 454 dataset here, but when I put that together with my Illumina data, I got worse assemblies with it. I assumed, like Sebastian mentioned, that this could be due to the homopolymer errors. From: raw...@gmail.com [raw...@gmail.com] Sent: Fri

[Denovoassembler-users] Number of Singletons (Reads that didn't assemble?) Does Ray calculate this stat?

2013-12-06 Thread Rick White
Hello, It would be important to know one way or another. Does it? Cheers Rick -- Richard Allen White III M.S. PhD Candidate - Suttle Lab Department of Microbiology & Immunology The University of British Columbia Vancouver, BC, Canada cell. 604-440-5150 http://www.ocgy.ubc.ca/~suttle/

Re: [Denovoassembler-users] Ion Torrent + Illumina Hybrid Assembly on Ray

2013-12-06 Thread raw937
Hello, Yes, I tried both. When I put Ion torrent reads with illumina or do just Ion torrent reads I get terrible assemblies. I am more of a fan of illumina but I have the Ion Torrent data and would like to get something out of it. Also, any thoughts for 454 and Illumina Hybrid assembly? I get o

Re: [Denovoassembler-users] Spelling of additional options not properly checked

2013-12-06 Thread Sébastien Boisvert
On 22/11/13 03:18 AM, Hornung, Bastian wrote: > Okay, email header sounds probably more scaring than it is ^^. > I just checked with what options my assembly is running (...SOP...), and > noticed that all my recent runs were with "-wirte-kmers", instead of > "-write-kmers" (yeah, stupid typo). >

Re: [Denovoassembler-users] Ion Torrent + Illumina Hybrid Assembly on Ray

2013-12-06 Thread Sébastien Boisvert
On 26/11/13 02:22 PM, Rick White wrote: > I have some 200bp Ion Torrent Mate Pair data, Paired 250x2 MiSeq reads and > have merged the overlapping reads from the Illumina data. > > When I try to run standard parameters in Ray the assemblies are worse with > the addition of the Ion Data. > > Do yo

Re: [Denovoassembler-users] Ray on x86 seg fault: Address not mapped (Vertex::addRead)

2013-12-06 Thread Sébastien Boisvert
On 02/12/13 07:32 PM, Jeff Tan wrote: > Hi all, > > We're seeing this seg fault occur in Vertex.cpp, it seems, using Ray 2.3.0 on > x86 launched via Slurm: Do you have a tail of stdout. Maybe it is related to what Matthew MacManes is seeing too. > > [jtan@barcoo-m barcoo]$ grep 27960 slurm-3497

Re: [Denovoassembler-users] _pmiu_daemon(SIGCHLD) segfault using Ray version 2.3.1-devel

2013-12-06 Thread MacManes, Matthew
This is Ray version 2.3.1-devel. I pulled from github a couple of days ago. Mat _ Matthew MacManes, Ph.D. University of New Hampshire I Assistant Professor Department of Molecular, Cellular, & Biomedical Sciences Durham, NH 03824 Phone: 603-862-4052 I Twit

Re: [Denovoassembler-users] _pmiu_daemon(SIGCHLD) segfault using Ray version 2.3.1-devel

2013-12-06 Thread Sébastien Boisvert
On 05/12/13 05:19 PM, MacManes, Matthew wrote: > Hi All, > > Having problems with segfault on a Cray XE6m-200 using 480 ranks: Looks like > 'Selection of optimal read markers’ had just finished. Which Ray version are you using ? > > error code: >> _pmiu_daemon(SIGCHLD): [NID 00285] [c1-0c2s1n3]

Re: [Denovoassembler-users] Ray is crashing for no apparent reason

2013-12-06 Thread Sébastien Boisvert
On 01/12/13 04:12 AM, Sucheta Tripathy wrote: > > I am trying to run Ray for a while now on our Illumina data. It is run in > parallel mode and memory is not a constraint now because we have a cluster > with 16 X 16 GB memory in each node. > The problem is this: tmp1 and tmp2 don't contain the s

Re: [Denovoassembler-users] Memory Limitations in RAY

2013-12-06 Thread Sébastien Boisvert
On 02/12/13 07:43 PM, Jeff Tan wrote: >> In the tests I did on this platform, I found that in general >> Ray needed between 400 MiB and 1 GiB of RAM per MPI rank. > > We must be doing something wrong then, because we were crashing out with > debug messages indicating over 2 GB in some ranks at leas

Re: [Denovoassembler-users] make on Cray XE system

2013-12-06 Thread Sébastien Boisvert
On 02/12/13 12:00 PM, MacManes, Matthew wrote: > Hello, > > Does anybody have experience using Ray on a Cray XE system? > > make MAXKMERLENGTH=96 >CXX code/application_core/ray_main.o > make: mpicxx: Command not found > For example, mpicxx invokes "g++ -I/usr/include/openmpi-x86_64 -pthread -m

Re: [Denovoassembler-users] Biological Abundance tests

2013-12-06 Thread Sébastien Boisvert
On 26/11/13 04:18 AM, Francesco Strozzi wrote: > Hi all, > I would like to raise a question to this point of the Biological Abundances > estimation. > How can we calculate the proportion of information that is not classified by > Ray Meta i.e. that part of the assembled information which is not c