[Denovoassembler-users] Ray Assembly with Mate Pairs?

2014-01-07 Thread Rick White
Hello, Is there a way to assembly long 20kb mate pair libraries on Ray? Both used the MiSeq 250x2 bp technology. Cheers Rick -- Rapidly troubleshoot problems before they affect your business. Most IT organizations don't

[Denovoassembler-users] Number of Singletons (Reads that didn't assemble?) Does Ray calculate this stat?

2013-12-06 Thread Rick White
Hello, It would be important to know one way or another. Does it? Cheers Rick -- Richard Allen White III M.S. PhD Candidate - Suttle Lab Department of Microbiology & Immunology The University of British Columbia Vancouver, BC, Canada cell. 604-440-5150 http://www.ocgy.ubc.ca/~suttle/

[Denovoassembler-users] Ion Torrent + Illumina Hybrid Assembly on Ray

2013-11-26 Thread Rick White
I have some 200bp Ion Torrent Mate Pair data, Paired 250x2 MiSeq reads and have merged the overlapping reads from the Illumina data. When I try to run standard parameters in Ray the assemblies are worse with the addition of the Ion Data. Do you have parameters that could help me out? Cheers Rick

Re: [Denovoassembler-users] Adding Coverage information for MG-RAST upload from RAY output? (Quick awk, sed or other).

2013-11-21 Thread Rick White
Hello Sebastien, Could you suggest a way to do it? Cheers Rick On Thu, Nov 21, 2013 at 1:20 PM, Sébastien Boisvert < sebastien.boisver...@ulaval.ca> wrote: > On 21/11/13 01:43 PM, Rick White wrote: > >> Hello, >> >> For MG-RAST upload it has two problems fro

[Denovoassembler-users] Adding Coverage information for MG-RAST upload from RAY output? (Quick awk, sed or other).

2013-11-21 Thread Rick White
Hello, For MG-RAST upload it has two problems from Ray outputs. The space between contig and number with unique sequence number for example. >contig-300 508 nucleotides And, it needs coverage information for >sequence_number_1_*[cov=2]* CTAGCGCACATAGCATTCAGCGTAGCAGTCACTAGTACGTAGTACGTACC >seq

[Denovoassembler-users] Mix up in MPI libraries for ubuntu 12.04 (and below)

2013-07-07 Thread Rick White
--Note for ubuntu users-- If you install sudo apt-get install libcr-dev mpich2 mpich2-doccd It will cause problems with you compile your Ray install with mpich2 --To avoid to this-- sudo apt-get install -y autotools-dev g++ build-essential sudo apt-get remove libcr-dev mpich2 mpich2-doccd sudo a

Re: [Denovoassembler-users] Fwd: Ray v2.2.0 stopped working - libmpichcxx.so.1.2 error

2013-07-05 Thread Rick White
form up and running on the Ray Dev version same problem. Still confused on how to fix it. Cheers and many thanks Rick On Fri, Jul 5, 2013 at 6:16 AM, Sébastien Boisvert < sebastien.boisver...@ulaval.ca> wrote: > On 04/07/13 04:29 PM, Rick White wrote: > >> >> &g

Re: [Denovoassembler-users] Error after reinstall. Removed libmpichcxx.so.1.2 but now no memory not running correctly

2013-07-04 Thread Rick White
What is the command you are using to launch Ray? > > Pier-Luc > Le 2013-07-04 18:51, Rick White a écrit : > > Hello again, > > I am confused on how to fix this problem in that it is not > multi-threading any more? > > As I don't understand how it could stop all the

Re: [Denovoassembler-users] Error after reinstall. Removed libmpichcxx.so.1.2 but now no memory not running correctly

2013-07-04 Thread Rick White
gt; git clone https://github.com/sebhtml/ray.git > > Pier-Luc > > > Le 2013-07-04 18:26, Rick White a écrit : > > Hello Pier-luc, > > > Normally, when I run the script I get more then one core running. But, > currently only one core is running at a time cau

Re: [Denovoassembler-users] Error after reinstall. Removed libmpichcxx.so.1.2 but now no memory not running correctly

2013-07-04 Thread Rick White
gt; to help you we need a little more information. > Which version of Ray are you using? > What are your system specs? > What is the size of your sample? > > Pier-Luc > > Le 2013-07-04 17:36, Rick White a écrit : > > Hello again, > > Fun continues. The reinsta

[Denovoassembler-users] Error after reinstall. Removed libmpichcxx.so.1.2 but now no memory not running correctly

2013-07-04 Thread Rick White
Hello again, Fun continues. The reinstall removed the libmpichcxx.so.1.2 error. But, now it isn't running correctly and is running out of memory error Critical exception: The system is out of memory, returned NULL. Requested -54534904 bytes of type RAY_MALLOC_TYPE_BLOOM_FILTER HELP! -- Richard

[Denovoassembler-users] Fwd: Ray v2.2.0 stopped working - libmpichcxx.so.1.2 error

2013-07-04 Thread Rick White
Hello Sebastien, (Ray users!) I had ray working quite well on our server then all the sudden it stopped. Worked fine yesterday. I get this error Ray: error while loading shared libraries: libmpichcxx.so.1.2: cannot open shared object file: No such file or directory I used sudo apt-get install in

Re: [Denovoassembler-users] Ray Cluster MPI error in Running?

2013-05-24 Thread Rick White
On Fri, May 24, 2013 at 11:39 AM, Rick White wrote: > It appeared the install went well but then I got this error on my ubuntu > server. > > cannot connect to local mpd (/tmp/mpd2.console_labop); possible causes: > 1. no mpd is running on this host > 2. an mpd is runni