Re: [EMBOSS] Help to build a motif for fzzpro

2012-10-19 Thread Peter Rice
On 19/10/2012 10:33, Dr. Josef Maier - IStLS wrote: Hello Angus, you could use preg with following pattern as written in regular expressions: ([KRH][^DE][^DE][^DE])|([^DE][KRH][^DE][^DE])|([^DE][^DE][KRH][^DE])|([^DE][^DE][^DE][KRH]) Alternatively you could search with four different PROSITE-st

Re: [EMBOSS] Help to build a motif for fzzpro

2012-10-19 Thread Guy Bottu
Dear Aengus, I have an idea of how to do it. You must of course complete the motif/pattern as much as possible because with just {DE}(4) you will find much to much unless you search only a few sequences. You must run the program fuzzpro with parameter -rformat=listfile. You will obtain

Re: [EMBOSS] Help to build a motif for fzzpro

2012-10-19 Thread Dr. Josef Maier - IStLS
s regular expressions. Best regards, Josef Josef Maier, IStLS Information Services to Life Science   > Date: Thu, 18 Oct 2012 15:52:44 +0100 > From: Aengus Stewart > Subject: [EMBOSS] Help to build a motif for fzzpro > To: emboss@lists.open-bio.org > Message-ID: <508017bc.80...@c

[EMBOSS] Help to build a motif for fzzpro

2012-10-18 Thread Aengus Stewart
Hi all, I have been asked to build a motif and the first part of the motif is. "The first 4 AAs must not be acidic and 1 of them should be basic" I am not sure how to do this. So far I have {DE}(4) I am thinking I run with this and then post-filter the fuzzpro file? Any ideas greatfully