[galaxy-dev] Galaxy September 7, 2012 Distribution & News Brief

2012-09-07 Thread Jennifer Jackson
*Galaxy September 7, 2012 Distribution & News Brief* http://wiki.g2.bx.psu.edu/DevNewsBriefs/2012_09_07 /*Highlights:*/ * *NCBI BLAST+ has moved* from the Galaxy distribution to the Galaxy's Main Tool Shed. * *Tool Shed* *is now running Mercurial version 2.2.3*. Many updates an

Re: [galaxy-dev] Automatic installation of third party dependancies

2012-09-07 Thread Greg Von Kuster
Hello Lance, See my inline comments. On Sep 4, 2012, at 3:15 PM, Lance Parsons wrote: > I've put together a tool wrapper for the htseq-count script that is part of > the HTSeq python package and uploaded that to the tool shed. However, I have > discovered that the tool dependancies do not in

[galaxy-dev] cuffdiff output: FPKM value zero

2012-09-07 Thread suzan katie
Hello everyone, I am comparing two samples (control and treated) paired end RNA Seq data. In the cuffdiff output I have noticed that few genes have zero FPKM value in one sample and other sample has significant FPKM value. I want to identify uniquely expressed genes identified only in one sample (

Re: [galaxy-dev] Toolsheds and job runners

2012-09-07 Thread Greg Von Kuster
Hi Paul, Since you are using one config file/one database, you just need to manually restart the job runner after installing tools (assuming your servers are configured correctly). The old way of having two config files, one for the web front ends and one for the SINGLE job runner is no longer

Re: [galaxy-dev] Adding tool to Galaxy

2012-09-07 Thread Akshay Bhat
Hi, Please follow the instructions on this page http://wiki.g2.bx.psu.edu/Admin/Tools/Add%20Tool%20Tutorial Best regards Akshay On Sep 7, 2012, at 3:01 PM, kauerb...@comcast.net wrote: > Hello, > > Can anyone tell me how to add a tool to Galaxy, to be shown on the panel on > the left?

[galaxy-dev] Adding tool to Galaxy

2012-09-07 Thread kauerbach
Hello, Can anyone tell me how to add a tool to Galaxy, to be shown on the panel on the left? In particular, I'd like to add the 'cuffmerge' tool to the cufflinks (RNA seq)  group under NGS:RNA Analysis. Other cufflinks tools are there (cufflinks,cuffcompare,cuffdiff), but not cuffmerge.

[galaxy-dev] Temporary directories

2012-09-07 Thread Joachim Jacob
Hi all, Just to share some experiences about temporary directories. - System /tmp is best put on a separate partition, with nosuid and noexec set. In /etc/fstab for example UUID=e35e1...d3/tmp ext4 noexec,nosuid,noatime,nodiratime0 0 - /tmp needs to be big, because it is used as t