Re: [galaxy-dev] galaxy-dev Digest, Vol 100, Issue 10

2014-10-17 Thread Iry Witham
? (Dannon Baker) 8. Re: Anyone else having issues with cloudman cloudlaunch? (Chris Dagdigian) 9. GATK2 java version related issues (Iry Witham) 10. Why does Galaxy CloudMan 2.3 (ami-a7dbf6ce) run the boinc client? (Chris Dagdigian) 11. Re: GATK2 java version related issues

Re: [galaxy-dev] galaxy-dev Digest, Vol 100, Issue 15

2014-10-17 Thread Iry Witham
for Galaxy extra_files_path (Jim Johnson) 7. snpEff and java issue(Iry Witham) 8. Re: HOWTO share tool parameter settings? (Lukasse, Pieter) 9.Help with Galaxy server migration (Sarah Diehl) 10. Re: Help with Galaxy server migrtion (John Chilton) 11. Login issue with a nginx proxy (Alexandre

[galaxy-dev] snpEff and java issue

2014-10-15 Thread Iry Witham
Hi Team, I had manually installed the latest version of snpEff based on the recommendation of Pablo and after modifying the XML files I had a working version of snpEff that produced a vcf. However, this morning I reran my workflow and it is failing again, but this time I am getting the

[galaxy-dev] GATK2 java version related issues

2014-10-10 Thread Iry Witham
Hi Team, I am sorry to be a pest, but I am under-the-gun to get this resolved. I have followed the instructions for installing GATK2 on my cloud VM. After doing that I discovered that the version of java running on the VM was v1.6.x and GATK2 required v1.7.0x. I removed version 6 and

Re: [galaxy-dev] GATK2 java version related issues

2014-10-10 Thread Iry Witham
there is no issue with the wrong java version running on additional nodes. Thanks, Iry From: Daniel Blankenberg d...@bx.psu.edumailto:d...@bx.psu.edu Date: Friday, October 10, 2014 3:16 PM To: Iry Witham iry.wit...@jax.orgmailto:iry.wit...@jax.org Cc: galaxy-dev@lists.bx.psu.edumailto:galaxy-dev

[galaxy-dev] GATK2 reporting Invalid argument value '/mnt/galaxy/tools/gatk2/bin/' at position 8.

2014-10-09 Thread Iry Witham
Hi Team, I have installed GATK2 into a galaxy cloud instance to use the Unified Genotyper for our sequencing course. However, when I run the tool I get the following error message: Dataset 41: Unified Genotyper on data 8 and data 13 (VCF) Tool execution generated the following error message:

[galaxy-dev] snpEf dataset generation errors

2014-10-09 Thread Iry Witham
I am trying to resolve an issue with snpEff 3.4 running on a cloudman instance of galaxy. The instance is invoking the following command and failing: java -Xmx6G -jar $SNPEFF_JAR_PATH/snpEff.jar eff -c $SNPEFF_JAR_PATH/snpEff.config -i vcf -o vcf -upDownStreamLen 5000 -spliceSiteSize 1 -stats

Re: [galaxy-dev] Cloudman indices installation/configuration

2014-10-07 Thread Iry Witham
fill in additional information about your genomes and annotations in the gatk2_picard_index.loc and gatk2_annotations.txt. You can find them in the tool-data/ Galaxy directory. From: Enis Afgan afg...@gmail.commailto:afg...@gmail.com Date: Saturday, October 4, 2014 6:10 AM To: Iry Witham iry.wit

[galaxy-dev] Cloudman indices installation/configuration

2014-10-02 Thread Iry Witham
Hi Team, I have a new instance of galaxy cloudman running and have added tools from the toolshed to it. When I attempt to run tools like sam-to-bam or any gatk tool I am prompted for a reference genome. However, indices/references not available for these tools. I have added the following

Re: [galaxy-dev] Cloudman indices installation/configuration

2014-10-02 Thread Iry Witham
modifying? Thanks, Iry From: Daniel Blankenberg d...@bx.psu.edumailto:d...@bx.psu.edu Date: Thursday, October 2, 2014 1:57 PM To: Iry Witham iry.wit...@jax.orgmailto:iry.wit...@jax.org Cc: galaxy-dev@lists.bx.psu.edumailto:galaxy-dev@lists.bx.psu.edu galaxy-dev@lists.bx.psu.edumailto:galaxy-dev

[galaxy-dev] Cloudman installed tools missing referneces

2014-09-26 Thread Iry Witham
Hi Team, I have a new instance of galaxy cloudman running on AWS and when I go to run some of the tools I have installed like SAM-to-BAM it requires a reference genome, but none is available. This is SAM-to-BAM version 1.1.4. This is the first tool I have found this to be an issue so far.

[galaxy-dev] Join tool failure

2014-09-04 Thread Iry Witham
Hi Team, I have upgraded to the latest galaxy distribution on my test server. Now I am running into issues and can't figure this one out. I am attempting to run the join.py tool to join two datasets at specified fields. I am getting the following error: Traceback (most recent call last):

Re: [galaxy-dev] Upload file not working with new install

2014-08-18 Thread Iry Witham
path. Thanks, Iry Sent from my iPhone On Aug 18, 2014, at 5:08 PM, Nate Coraor n...@bx.psu.edumailto:n...@bx.psu.edu wrote: On Aug 18, 2014, at 9:55 AM, Iry Witham iry.wit...@jax.orgmailto:iry.wit...@jax.org wrote: Hi John, I was able to work through this since it was related to pbs

Re: [galaxy-dev] Upload file not working with new install

2014-08-15 Thread Iry Witham
: Thursday, August 14, 2014 12:48 PM To: Iry Witham iry.wit...@jax.orgmailto:iry.wit...@jax.org, galaxy-dev@lists.bx.psu.edumailto:galaxy-dev@lists.bx.psu.edu galaxy-dev@lists.bx.psu.edumailto:galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] Upload file not working with new install No sure

Re: [galaxy-dev] Upload file not working with new install

2014-08-15 Thread Iry Witham
some questions - Are you using a proxy - if so which one? Can you attach the configuration? Is the upload.xml tool in your tool_conf.xml? Have you made modifications to it? -John On Fri, Aug 15, 2014 at 10:47 AM, Iry Witham iry.wit...@jax.org wrote: I have checked and everything appears correct

[galaxy-dev] Upload file not working with new install

2014-08-14 Thread Iry Witham
Hi Team, I have installed a fresh version of Galaxy and am having an issue with the 'Upload File' tool. When I attempt to use it it just sits and spins. No data gets uploaded. I have looked through the universe.wsgi.ini file and cannot find anything there that stands out. Can you point me

[galaxy-dev] Issues with dependencies for toolshed packages

2014-08-06 Thread Iry Witham
Hi Team, I have been trying to install package_snpEff_3_5 from the Galaxy main tool shed and package_snpEff_3_6 from the Galaxy test tool shed and have the same issue. Once the installer completes it shows that the dependency snpEff.x.x was not installed. When I try to install the dependency

Re: [galaxy-dev] Issues with dependencies for toolshed packages

2014-08-06 Thread Iry Witham
...@bx.psu.edumailto:g...@bx.psu.edu Date: Wednesday, August 6, 2014 12:34 PM To: Iry Witham iry.wit...@jax.orgmailto:iry.wit...@jax.org Cc: galaxy-dev@lists.bx.psu.edumailto:galaxy-dev@lists.bx.psu.edu galaxy-dev@lists.bx.psu.edumailto:galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] Issues

Re: [galaxy-dev] Issues with dependencies for toolshed packages

2014-08-06 Thread Iry Witham
/galaxy-setup/shed_tools/tool_dependencies/snpEff/3.4/iuc/package_snpeff_3_4/af7ff8187d09/env.sh ; fi Thanks, Iry From: bjoern.gruen...@googlemail.commailto:bjoern.gruen...@googlemail.com bjoern.gruen...@gmail.commailto:bjoern.gruen...@gmail.com Date: Wednesday, August 6, 2014 1:16 PM To: Iry Witham

[galaxy-dev] Fresh galaxy install fails to start

2014-07-17 Thread Iry Witham
Hi Dev Team, I am attempting to get a new/clean installation running and am having difficulty. I did a clone from https://bitbucket.org/galaxy/galaxy-dist and made my needed configuration changes for my environment. I attempted to start it and needed to do the database upgrade via sh

[galaxy-dev] Continued issue fetching eggs

2014-01-07 Thread Iry Witham
Hi Team, Quite some time back I posted that I was unable to upgrade my local galaxy instance due to issues fetching eggs. I have not been able to get this resolved and was wondering if I could get some help. The error I get is: python scripts/fetch_eggs.py Warning: setuptools (a dependent

[galaxy-dev] Egg distribution issue

2013-11-05 Thread Iry Witham
Hi Dev Team, I have run into an egg distribution issue. I had performed an upgrade late last month and am experiencing issues fetching eggs. I am getting the following error message: galaxy@jaxgalaxydev01:/hpcdata/galaxy-dev/galaxy-setup/galaxy-dist python ./scripts/fetch_eggs.py Fetched

Re: [galaxy-dev] configuring auth_ldap for galaxy

2013-10-01 Thread Iry Witham
...@msi.umn.edu Date: Wednesday, September 18, 2013 7:49 AM To: Iry Witham iry.wit...@jax.org Cc: galaxy-dev@lists.bx.psu.edu galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] configuring auth_ldap for galaxy You have to compile nginx with the auth-ldap module. Have you done this? I am doing using

[galaxy-dev] configuring auth_ldap for galaxy

2013-09-18 Thread Iry Witham
of parent of /usr/local/nginx/sbin/nginx: 1 failed Any help would be appreciated. Thanks, Iry Witham The information in this email, including attachments, may

[galaxy-dev] Galaxy ego fetching error (Mac OS X 10.8)

2013-07-16 Thread Iry Witham
. Thanks, Iry Witham The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible

[galaxy-dev] Resizing galaxy cloudman /mnt/galaxyData partition

2013-05-06 Thread Iry Witham
what I need to? Thanks, Iry Witham The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible

Re: [galaxy-dev] Resizing galaxy cloudman /mnt/galaxyData partition

2013-05-06 Thread Iry Witham
Hi Dannon, I will give it a shot. Are you familiar with the load balancer and how to make it work? Or the auto scaling feature? Thanks, Iry From: Dannon Baker dannon.ba...@gmail.commailto:dannon.ba...@gmail.com Date: Monday, May 6, 2013 2:06 PM To: Iry Witham iry.wit

Re: [galaxy-dev] Resizing galaxy cloudman /mnt/galaxyData partition

2013-05-06 Thread Iry Witham
: Dannon Baker dannon.ba...@gmail.commailto:dannon.ba...@gmail.com Date: Monday, May 6, 2013 2:06 PM To: Iry Witham iry.wit...@jax.orgmailto:iry.wit...@jax.org Cc: galaxy-dev@lists.bx.psu.edumailto:galaxy-dev@lists.bx.psu.edu galaxy-dev@lists.bx.psu.edumailto:galaxy-dev@lists.bx.psu.edu Subject: Re

Re: [galaxy-dev] Resizing galaxy cloudman /mnt/galaxyData partition

2013-05-06 Thread Iry Witham
I was not clear how the load balancer worked in this instance, but my goal was to improve performance. I am not sure this was the correct use, but it seemed to work. From: Dannon Baker dannon.ba...@gmail.commailto:dannon.ba...@gmail.com Date: Monday, May 6, 2013 4:12 PM To: Iry Witham iry.wit

Re: [galaxy-dev] Resizing galaxy cloudman /mnt/galaxyData partition

2013-05-06 Thread Iry Witham
I did add nodes about the same time I setup load balancing so that was likely the fix. I will definitely set the head node not to run jobs. Thanks, Iry From: Dannon Baker dannon.ba...@gmail.commailto:dannon.ba...@gmail.com Date: Monday, May 6, 2013 4:19 PM To: Iry Witham iry.wit

[galaxy-dev] MPileup for cloudman instance

2013-05-02 Thread Iry Witham
Hi All, I have noticed that there is no Mpileup available in either of the tool sheds. Is there a simple way to install it in my instance? Thanks, Iry The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you

[galaxy-dev] SnpEff Ver 3.1 produces 'no peek' results

2013-05-01 Thread Iry Witham
(SnpEffCmdEff.java:564) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.run(SnpEff.java:276) at ca.mcgill.mcb.pcingola.snpEffect.commandLine.SnpEff.main(SnpEff.java:75) Any ideas? Thanks, Iry Witham The information in this email, including attachments, may

Re: [galaxy-dev] Installing FreeBayes via Toolshed: Tool dependency installation error

2013-05-01 Thread Iry Witham
FILTER INFOFORMAT unknown Iry From: Dannon Baker dannon.ba...@gmail.commailto:dannon.ba...@gmail.com Date: Tuesday, April 30, 2013 1:06 PM To: Iry Witham iry.wit...@jax.orgmailto:iry.wit...@jax.org Cc: galaxy-dev@lists.bx.psu.edumailto:galaxy-dev@lists.bx.psu.edu galaxy-dev

Re: [galaxy-dev] Installing FreeBayes via Toolshed: Tool dependency installation error

2013-05-01 Thread Iry Witham
out of my wheelhouse, but did you verify that the reference it's trying to use is available? Does this same job work if you try it on galaxy main (if that's an option)? reference=/mnt/galaxyIndices/genomes/Hsapiens/hg19/seq/hg19.fa On Wed, May 1, 2013 at 8:41 AM, Iry Witham iry.wit

[galaxy-dev] Installing FreeBayes via Toolshed: Tool dependency installation error

2013-04-30 Thread Iry Witham
, Iry Witham The information in this email, including attachments, may be confidential and is intended solely for the addressee(s). If you believe you received this email by mistake, please notify the sender by return email as soon as possible

[galaxy-dev] Adding GATK tools to Galaxy Cloudman

2013-04-24 Thread Iry Witham
Hi Team, I am trying to get GATK tools added to my cloud instance and keep getting the following error: [Errno 2] No such file or directory: '/mnt/galaxyData/tmp/tmpszWRLP/GenomeAnalysisTKLite-2.3-9-gdcdccbb/GenomeAnalysisTK.jar'. Then when I try to install it again I get the following: All

[galaxy-dev] Setting up Cloudman instance

2013-03-22 Thread Iry Witham
and the instructions at http://wiki.galaxyproject.org/CloudMan and have had the same results. I have read a few posts of similar issues, but have not found a solution. Any suggestions will be helpful. Thanks, Iry Witham __ Scientific Applications Administrator

[galaxy-dev] GATK fails to create Java Virtual Machine

2012-11-09 Thread Iry Witham
not create the Java virtual machine. I have looked at the wrapper for java settings and restarted the Galaxy server, but am at a loss. Do you have any ideas? Thanks, Iry Witham Scientific Applications Administrator Scientific Computing Group Computational Sciences Dept. The Jackson Laboratory 600

[galaxy-dev] Error running cuffmerge

2012-10-19 Thread Iry Witham
, Iry Witham Scientific Applications Administrator Scientific Computing Group Computational Sciences Dept. The Jackson Laboratory 600 Main Street Bar Harbor, ME 04609 Phone: 207-288-6744 email: iry.wit...@jax.org The information in this email, including attachments, may be confidential

[galaxy-dev] Issues with database / metadata after upgrade

2012-08-29 Thread Iry Witham
Hi Team, I had performed an upgrade of my Galaxy server and had experienced an issue with some of the database / metadata files have been corrupted. I have found instances where the dataset exists, but cannot be detected or read by galaxy when attempting to analysis data from a history. In

Re: [galaxy-dev] Issue configuring reports_wsgi.ini

2012-08-24 Thread Iry Witham
Hans, I set the actual IP address and it works. Thanks, Iry On 8/24/12 8:07 AM, Hans-Rudolf Hotz h...@fmi.ch wrote: Hi Iry - try different port - try the actual IP address of your host Regards, Hans On 08/24/2012 01:52 PM, Iry Witham wrote: Hi Team, I am attempting to get

[galaxy-dev] Galaxy Reports no longer functions

2012-08-17 Thread Iry Witham
Hi Team, Since I upgraded to the latest galaxy-dist I can no longer get the reports interface to function. When I go to galaxy:9001 I get the following server error: An error occurred. See the error logs for more information. (Turn debug on to display exception reports here). The

[galaxy-dev] Strange Could not moveŠ. error

2012-08-02 Thread Iry Witham
Hi Dev-Team, I am experiencing a strange error that I cannot replicate on two identical instances of my Galaxy server. I have a tool that runs fine on my test instance and fails on my development instance. The servers are identically configured and running the same build. The handler.log

Re: [galaxy-dev] Strange Could not moveŠ. error

2012-08-02 Thread Iry Witham
scottmcma...@gatech.edumailto:scottmcma...@gatech.edu Date: Thursday, August 2, 2012 12:59 PM To: Iry Witham iry.wit...@jax.orgmailto:iry.wit...@jax.org Cc: galaxy-dev@lists.bx.psu.edumailto:galaxy-dev@lists.bx.psu.edu galaxy-dev@lists.bx.psu.edumailto:galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy

Re: [galaxy-dev] pbs_python issues

2012-07-13 Thread Iry Witham
, at 12:32 PM, Iry Witham wrote: I am having an issue with pbs_python loading, but not submitting jobs to the cluster. The first issue is that when I ran 'LIBTORQUE_DIR=/usr/local/lib/ python scripts/scramble.py ­e pbs_python' it failed to generate the pbs_python egg in the /galaxy-dist/eggs

Re: [galaxy-dev] pbs_python issues

2012-07-13 Thread Iry Witham
Will the runner0.log or web0.log be sufficient? If not what would you like? Iry On 7/13/12 2:23 PM, Nate Coraor n...@bx.psu.edu wrote: On Jul 13, 2012, at 9:05 AM, Iry Witham wrote: Hi Nate, I have set this as shown below: # The URL for the default runner to use when a tool

Re: [galaxy-dev] pbs_python issues

2012-07-11 Thread Iry Witham
galaxy-dev-requ...@lists.bx.psu.edu wrote: Message: 3 Date: Mon, 9 Jul 2012 17:24:25 + From: Iry Witham iry.wit...@jax.org To: Dorset, Daniel C daniel.dor...@vanderbilt.edu Cc: galaxy-dev@lists.bx.psu.edu galaxy-dev@lists.bx.psu.edu Subject: Re: [galaxy-dev] galaxy-dev Digest, Vol 73, Issue 10

Re: [galaxy-dev] galaxy-dev Digest, Vol 73, Issue 10

2012-07-09 Thread Iry Witham
meaningful. People will have some idea from the subject line if they should read it or not. 2. Not doing this greatly increases the number of emails that match search queries, but that aren't actually informative. Today's Topics: 1. pbs_python issues (Iry Witham) 2. Which css file does welcome.html

[galaxy-dev] pbs_python issues

2012-07-06 Thread Iry Witham
I am having an issue with pbs_python loading, but not submitting jobs to the cluster. The first issue is that when I ran 'LIBTORQUE_DIR=/usr/local/lib/ python scripts/scramble.py –e pbs_python' it failed to generate the pbs_python egg in the /galaxy-dist/eggs/ directory. Secondly I copied the

Re: [galaxy-dev] Server Error after fresh installation of Galaxy

2012-07-03 Thread Iry Witham
I am still experiencing issues getting my galaxy server to start since the upgrade. The logs are all getting this type of error: galaxy@galaxy2:/hpcdata/galaxy-test/galaxy-setup/galaxy-dist cat runner0.log python path is:

Re: [galaxy-dev] Server Error after fresh installation of Galaxy

2012-07-03 Thread Iry Witham
for galaxy-dist. 2) Swap to galaxy-central instead of -dist Taking a brief look at the migrations, #1 looks fairly safe, but as always -- back up the database first. -Dannon On Jul 3, 2012, at 1:03 PM, Iry Witham wrote: I am still experiencing issues getting my galaxy server to start since

Re: [galaxy-dev] Server Error after fresh installation of Galaxy

2012-06-27 Thread Iry Witham
(vernum)] KeyError: VerNum(101) It is getting closer, but I am not quite there yet. Thanks, Iry On 6/22/12 11:50 AM, Nate Coraor n...@bx.psu.edu wrote: On Jun 20, 2012, at 6:46 PM, Iry Witham wrote: I have recently performed a fresh installation of Galaxy utilizing the latest

[galaxy-dev] Galaxy-Development site error

2012-02-28 Thread Iry Witham
or unsupported form of compression. This happens with Firefox 3.6.27, but not with Crome or Safari. By default I use Firefox 3.6.x due to performance issues with the later versions. Thanks, Iry Witham Scientific Applications Administrator Scientific Computing Group Computational Sciences Dept

[galaxy-dev] Issues importing a shared workflow

2012-02-24 Thread Iry Witham
/workflow-constructed-from-history-chipseqworkflowwithcontrol How do I fix this issue? Are there settings that need to be modified on the users side? Thanks Iry Witham Scientific Applications Administrator Scientific Computing Group Computational Sciences Dept. The Jackson Laboratory 600 Main Street

Re: [galaxy-dev] [Newsletter] Re: Issues importing a shared workflow

2012-02-24 Thread Iry Witham
On 2/24/12 3:45 PM, Dannon Baker dannonba...@me.com wrote: How did the user share the workflow with you, via link or directly with your user? And which import option are you using? Does Clone in the workflow context menu work? -Dannon On Feb 24, 2012, at 3:25 PM, Iry Witham wrote: I have

[galaxy-dev] manage_db.sh upgrade fails after fresh install of Galaxy

2011-12-21 Thread Iry Witham
I have installed a new Galaxy server utilizing the November 18,2011 distribution on a SLES 11 VM and cannot get it to launch cleanly. I am running postgreSQL v. 8.3.9 to create my database. Once the database is created and running I launch my Galaxy server and within moments all of the PID

Re: [galaxy-dev] jquery script error

2011-11-14 Thread Iry Witham
tried it with Google Crome and Firefox 3.6.20. Thanks, Iry Witham ___ Please keep all replies on the list by using reply all in your mail client. To manage your subscriptions to this and other Galaxy lists, please use the interface at: http

[galaxy-dev] manage_db.sh upgrade error

2011-09-30 Thread Iry Witham
When I run sh manage_db.sh upgrade I get the following error: 2 - 3... Traceback (most recent call last): File ./scripts/manage_db.py, line 55, in module main( repository=repo, url=db_url ) File

[galaxy-dev] LookupError: No section 'main' (prefixed by 'server') found in config

2011-08-26 Thread Iry Witham
I have performed a new install of Galaxy for development and deployment purposes. However, when I run the run.sh script I get the following error: Traceback (most recent call last): File ./scripts/paster.py, line 34, in module command.run() File

Re: [galaxy-dev] Display BAM with IGV

2011-08-24 Thread Iry Witham
this with IGV support. Thanks, Iry On 8/15/11 2:46 PM, Nate Coraor n...@bx.psu.edu wrote: Iry Witham wrote: Hi Nate, I have checked the nginx.conf file and they comply to the documentation: # added to allow nginx to handle file downloads location /_x_accel_redirect

Re: [galaxy-dev] Display BAM with IGV

2011-08-15 Thread Iry Witham
the following: And the following to the [app:main] section of universe_wsgi.ini: nginx_x_accel_redirect_base = /_x_accel_redirect Iry On 8/15/11 10:46 AM, Nate Coraor n...@bx.psu.edu wrote: Iry Witham wrote: Hi Everyone, I have been tasked with getting the IGV working within our Galaxy instance

Re: [galaxy-dev] Display BAM with IGV

2011-08-15 Thread Iry Witham
On 8/15/11 2:46 PM, Nate Coraor n...@bx.psu.edu wrote: Iry Witham wrote: Hi Nate, I have checked the nginx.conf file and they comply to the documentation: # added to allow nginx to handle file downloads location /_x_accel_redirect/ { internal