Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Björn Grüning
Hi Vipin and Rui, please have at the toolshed and test toolshed before developing wrappers. For deseq2 there are already wrappers that are trying to solve your issues and it would be nice if you could join that effort. Help testing and contributing to it. Ross has a cool wrapper of edgeR, DESeq2

Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Joachim Jacob | VIB |
Hi Rui, I should provide a 'sample metadata table', a tabular file with one column the names of your samples, the other column the associated metadata (e.g. type). This is how I've done it in my DESeq2 wrapper. :-) Feel free to check the interface at

Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Peter Cock
Wow - that makes at least five Deseq2 wrappers for Galaxy available or in progress :( Bjoern's wrapper on the (test) tool shed, http://testtoolshed.g2.bx.psu.edu/view/bgruening/deseq2 Ross' combined wrapper for edgeR, DESeq2 and voom in one tool:

Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Joachim Jacob | VIB |
It is not necessarily bad. If we all test each others tools (dependencies, interface, output,...) we might get to the best of all worlds :-) (I have planned to test Björns wrapper) One of the points of differentiation is also how 'integrated' a tool needs to be: e.g. Ross' tool for diff

Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Peter Cock
On Wed, Sep 11, 2013 at 10:12 AM, Joachim Jacob | VIB | joachim.ja...@vib.be wrote: It is not necessarily bad. If we all test each others tools (dependencies, interface, output,...) we might get to the best of all worlds :-) (I have planned to test Björns wrapper) One of the points of

Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Bjoern Gruening
Am Mittwoch, den 11.09.2013, 10:21 +0100 schrieb Peter Cock: On Wed, Sep 11, 2013 at 10:12 AM, Joachim Jacob | VIB | joachim.ja...@vib.be wrote: It is not necessarily bad. If we all test each others tools (dependencies, interface, output,...) we might get to the best of all worlds :-) (I

Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Vipin TS
Yes I am agreeing on all statements. Let me finish my package and will add in one of the toolshed by today evening. I also willing to test other wrappers to improve the additional parameter settings. Tool wrappers Work In Progress from my side: DEXSeq edgeR Scripture STAR aligner SAFT multi

Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Ross
Vipin, Search works pretty well on the toolshed - I'd recommend a search for (eg) 'star' before writing yet another rnastar toolshed wrapper and dependency package? There's an installable fubar package_rnastar and an rnastar wrapper based on Jeremy's code mentioned below - but working AFAIK - at

Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread Jeremy Goecks
STAR aligner I have a partial STAR wrapper that may be useful: https://bitbucket.org/jgoecks/jeremys-code/src/d7096c132657420f08b540c6954b947f648aac80/galaxy/tool-wrappers/star.xml?at=default Whether you use this code or not, I do have one request: please put the output in BAM rather than

Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread ruiwang.sz
Hi Joachim, Thanks for the info! but I could not register on your instance...and thus could not check your wrapper. :-( Please help! Thanks, Rui On Wed, Sep 11, 2013 at 12:59 AM, Joachim Jacob | VIB | joachim.ja...@vib.be wrote: Hi Rui, I should provide a 'sample metadata table', a

Re: [galaxy-dev] Deseq2 wrapper question

2013-09-11 Thread ruiwang.sz
Hey Guys, It's great to receive so many responses! Thank you all for the information... and let me check out each of the masterpiece. :-) Best, Rui On Wed, Sep 11, 2013 at 1:56 AM, Peter Cock p.j.a.c...@googlemail.comwrote: Wow - that makes at least five Deseq2 wrappers for Galaxy available

[galaxy-dev] Deseq2 wrapper question

2013-09-10 Thread ruiwang.sz
Hi All, we are making a wrapper for deseq2, and there is a step where control/experimental conditions need to be determined. For example, it would be a column like the following in the input file Type CTC CTC LM LM PT PT and we want the select list to contain CTC LM PT In other words, we want

Re: [galaxy-dev] Deseq2 wrapper question

2013-09-10 Thread Vipin TS
Hello Rui, We have DESeq2 wrapper in our oqtans package and you will find this at https://github.com/ratschlab/oqtans_tools/tree/master/DESeq2 I am currently creating the associated tool_dependency.xml file for dumping the package in galaxy toolshed. otherwise it requires some manual effort to