Re: [galaxy-user] Enis: [galaxy-bugs] Security Groups int the cloud

2011-07-19 Thread Enis Afgan
Hi Luciano, Did you first create the galaxyWeb security group? Instructions are available on usegalaxy.org/cloud under Step 1. Enis On 7/18/11 2:37 PM, Luciano Cosme wrote: Hi, I am trying to run Galaxy in the cloud and I am following your screencast. I am having a problem when I need

Re: [galaxy-user] BAM files loading and mandatory grooming

2011-07-19 Thread Louise-Amélie Schmitt
Hello Jennifer, and thanks for your answer Would you be able to help with a bit more detail and testing? #2 sounds like it may be the issue, but without knowing more right now, I'll provided the next troubleshooting steps. Yeah, I realise I was not very clear, sorry about that. 1 - this

Re: [galaxy-user] using files produced by Barcode Splitter

2011-07-19 Thread Jim Johnson
I've had users make a similar request for the split files to appear in their history. I made my own wrapper to enable this behavior. It presents a list of barcodes from the barcode file input. Any barcodes the user selects will have the resulting files copied to the users history as new

Re: [galaxy-user] using files produced by Barcode Splitter

2011-07-19 Thread Jim Johnson
Jeremy, Usually, the users saved the files to their local machine by clicking on the links, and then uploaded them again to the galaxy server. It can be possible to copy the link location, and paste it into the upload tools URL textarea. Ideally, it would be nice for the tool developer to be

[galaxy-user] Tophat error: broken pipe

2011-07-19 Thread Song Li
Hello everyone, I was trying to run tophat in a local version of galaxy, but I got the following error: gzip: stdout: Broken pipe [Tue Jul 19 14:10:36 2011] Processing bowtie hits Error: could not open pipe gzip -cd ./tophat_out/tmp/left_kept_reads_missing.fq.z It seems that when tophat is

Re: [galaxy-user] using files produced by Barcode Splitter

2011-07-19 Thread Jeremy Coate
Ah ha - thanks. I was trying to click the link, but it kept crashing, presumably because the files are too big. The URL method seems to work, though. I agree that it would be nice if there was a way to bump the files directly into your history without the download/upload steps. Thanks again for

Re: [galaxy-user] Cufflinks reporting FPKM values of all zeroes (0)

2011-07-19 Thread Jennifer Jackson
Hello David, There is most likely some mismatch between the input data. Some things to check: First, double check that the identifiers in the reference genome exactly match those in the RefGene gtf file and modify if necessary. Second, make certain that your RefGene file is sorted the same

Re: [galaxy-user] Tophat error: broken pipe

2011-07-19 Thread Jennifer Jackson
Hello Song Li, The file extension seems to be a mismatch. file.gz - gzip utility Exploring the use of gunzip or zcat are options to restore a file.z. Hopefully this helps, Jen Galaxy team On 7/19/11 11:52 AM, Song Li wrote: Hello everyone, I was trying to run tophat in a local version of